PMID- 6438323
OWN - NLM
STAT- MEDLINE
DCOM- 19850114
LR  - 20111117
IS  - 0022-2615 (Print)
IS  - 0022-2615 (Linking)
VI  - 18
IP  - 3
DP  - 1984 Dec
TI  - Evaluation of mycobacterial antigens in an enzyme-linked immunosorbent assay 
      (ELISA) for the serodiagnosis of tuberculosis.
PG  - 309-18
AB  - Five mycobacterial antigens were compared in an enzyme-linked immunosorbent assay 
      (ELISA) for the serodiagnosis of tuberculosis. The antigens studied were an 
      unheated sterile culture filtrate of Mycobacterium tuberculosis, tuberculin 
      purified protein derivative (PPD) from M. tuberculosis (PPDa), purified 
      cytoplasmic protein antigens 5 and 6 from M. tuberculosis, and a PPD prepared 
      from M. kansasi (PPDk). Multivariate analysis of variance showed that geometric 
      mean titres obtained with each of the antigens in ELISA were significantly 
      different in tuberculosis patients and in control groups. The covariation of the 
      ELISA results with the five antigens was highly interdependent. Analysis of 
      receiver operating characteristics revealed that the most accurate test was 
      obtained with antigen 5. M. tuberculosis PPD, M. tuberculosis antigen 6, and M. 
      tuberculosis culture filtrate were, in descending order, less accurate.
FAU - Benjamin, R G
AU  - Benjamin RG
FAU - Debanne, S M
AU  - Debanne SM
FAU - Ma, Y
AU  - Ma Y
FAU - Daniel, T M
AU  - Daniel TM
LA  - eng
GR  - AI-15102/AI/NIAID NIH HHS/United States
GR  - HL-07288/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Tuberculin)
SB  - IM
MH  - Antibodies, Bacterial/*analysis
MH  - Antigens, Bacterial/*immunology
MH  - *Enzyme-Linked Immunosorbent Assay
MH  - False Positive Reactions
MH  - Humans
MH  - *Immunoenzyme Techniques
MH  - Mycobacterium tuberculosis/*immunology
MH  - Nontuberculous Mycobacteria/immunology
MH  - Tuberculin/immunology
MH  - Tuberculosis, Pulmonary/*diagnosis/immunology
EDAT- 1984/12/01 00:00
MHDA- 1984/12/01 00:01
CRDT- 1984/12/01 00:00
PHST- 1984/12/01 00:00 [pubmed]
PHST- 1984/12/01 00:01 [medline]
PHST- 1984/12/01 00:00 [entrez]
AID - 10.1099/00222615-18-3-309 [doi]
PST - ppublish
SO  - J Med Microbiol. 1984 Dec;18(3):309-18. doi: 10.1099/00222615-18-3-309.