PMID- 6440008
OWN - NLM
STAT- MEDLINE
DCOM- 19850207
LR  - 20190825
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 21
IP  - 11
DP  - 1984 Nov
TI  - Immunogenicity of biotinylated hapten-avidin complexes.
PG  - 1055-60
AB  - The efficacy of avidin as a carrier for the generation of anti-hapten antibodies 
      was assessed in mice by immunization with complexes of avidin and synthetic 
      peptides containing biotin and an epsilon-dinitrophenyl (DNP) lysine residue. The 
      synthetic haptens were constructed with 0, 1 or 2 6-aminocaproyl groups as 
      spacers between the biotin and DNP-lysine moieties. Complexes without a spacer 
      did not induce anti-DNP antibody responses, while those with two spacers induced 
      stronger responses than those with only one spacer. However, the anti-DNP 
      responses to avidin-biotinylated hapten complexes were considerably weaker than 
      responses to a conventional hapten-protein conjugate (DNP-ovalbumin), and, like 
      "T-independent" antigens, failed to induce significant immunological memory. The 
      distribution of isotypes in the anti-DNP antibodies produced to 
      avidin-biotin-6-aminocaproyl-epsilon-DNP-lysine-alanine and DNP-ovalbumin was 
      similar, but the former antigen induced significantly lower levels of antibody in 
      (CBA/N X BALB/c) F1 male mice with the xid defect than in phenotypically normal 
      female littermates, and also induced significant responses in nu/nu mice, in 
      contrast to DNP-ovalbumin. These findings suggest that there is at least a 
      "T-independent" or "T-efficient" component in the response to avidin-biotin 
      complexes, perhaps due to the tetrameric structure of the molecule. Estimates of 
      the depth of the receptor site for biotin were obtained by using the complexes to 
      competitively inhibit the binding of anti-DNP antibody to plates coated with 
      DNP-protein. The findings were consonant with the data on immunogenicity 
      (capacity to induce anti-DNP antibody responses) and suggested that the receptor 
      site has a depth of 16-26 A.
FAU - Scott, D
AU  - Scott D
FAU - Nitecki, D E
AU  - Nitecki DE
FAU - Kindler, H
AU  - Kindler H
FAU - Goodman, J W
AU  - Goodman JW
LA  - eng
GR  - AI-05664/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Aminocaproates)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Haptens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Peptides)
RN  - 1405-69-2 (Avidin)
RN  - 6SO6U10H04 (Biotin)
RN  - 9006-59-1 (Ovalbumin)
RN  - K3Z4F929H6 (Lysine)
RN  - OF5P57N2ZX (Alanine)
SB  - IM
MH  - Alanine/immunology
MH  - Aminocaproates/immunology
MH  - Animals
MH  - Antibody Formation
MH  - Avidin/*immunology
MH  - Binding, Competitive
MH  - Biotin/*immunology
MH  - Dinitrobenzenes/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Haptens/*immunology
MH  - Immunoglobulin G/biosynthesis
MH  - Lysine/immunology
MH  - Macromolecular Substances
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Ovalbumin/*analogs & derivatives
MH  - Peptides/immunology
EDAT- 1984/11/01 00:00
MHDA- 1984/11/01 00:01
CRDT- 1984/11/01 00:00
PHST- 1984/11/01 00:00 [pubmed]
PHST- 1984/11/01 00:01 [medline]
PHST- 1984/11/01 00:00 [entrez]
AID - 10.1016/0161-5890(84)90115-9 [doi]
PST - ppublish
SO  - Mol Immunol. 1984 Nov;21(11):1055-60. doi: 10.1016/0161-5890(84)90115-9.