PMID- 6442509
OWN - NLM
STAT- MEDLINE
DCOM- 19850510
LR  - 20191031
IS  - 0176-6724 (Print)
IS  - 0176-6724 (Linking)
VI  - 258
IP  - 2-3
DP  - 1984 Dec
TI  - Purification and partial characterization of Fusobacterium necrophorum 
      hemagglutinin.
PG  - 232-41
AB  - Hemagglutinin (HAin) of Fusobacterium necrophorum was separated from the 
      bacterial cells by trypsinization-sonication, and purified by the gel filtration 
      on Sephadex G-100 column. The final product obtained from gel filtration gave one 
      precipitin line in the immunodiffusion gel and produced a single band in 
      polyacrylamide gel electrophoresis. The molecular weight of the HAin was 
      estimated to be about 19000 by sodium dodecyl sulfate polyacrylamide gel 
      electrophoresis. It was heat labile and comparatively rich in alanine, glutamine 
      and histidine. Electron microscopy observation revealed that the HAin was a 
      filamentous rod with 0.5-1.0 nm width or frequently showed a cluster form. The 
      hemagglutinability was inhibited by addition of albumins but not by sugars and 
      lipopolysaccharide. Anti HAin rabbit serum inhibited hemagglutination.
FAU - Nagai, S
AU  - Nagai S
FAU - Kanoe, M
AU  - Kanoe M
FAU - Toda, M
AU  - Toda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Zentralbl Bakteriol Mikrobiol Hyg A
JT  - Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical 
      microbiology, infectious diseases, virology, parasitology
JID - 8403032
RN  - 0 (Amino Acids)
RN  - 0 (Hemagglutinins)
SB  - IM
MH  - Amino Acids/analysis
MH  - Chromatography, Gel
MH  - Electrophoresis, Disc
MH  - Fusobacterium necrophorum/*analysis
MH  - Hemagglutination Tests
MH  - Hemagglutinins/analysis/immunology/*isolation & purification
MH  - Hot Temperature
MH  - Immunodiffusion
MH  - Molecular Weight
MH  - Sonication
EDAT- 1984/12/01 00:00
MHDA- 1984/12/01 00:01
CRDT- 1984/12/01 00:00
PHST- 1984/12/01 00:00 [pubmed]
PHST- 1984/12/01 00:01 [medline]
PHST- 1984/12/01 00:00 [entrez]
AID - 10.1016/s0176-6724(84)80041-3 [doi]
PST - ppublish
SO  - Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Dec;258(2-3):232-41. doi: 
      10.1016/s0176-6724(84)80041-3.