PMID- 6458330
OWN - NLM
STAT- MEDLINE
DCOM- 19820222
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 20
IP  - 23
DP  - 1981 Nov 10
TI  - Unexpected findings from target analysis of immunoglobulin E and its receptor.
PG  - 6589-94
AB  - The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were 
      irradiated with high-energy electrons. Loss of binding activity was measured for 
      each, and the size of the functional targets was assessed. In both cases, the 
      target size was substantially smaller than the covalent structure of the 
      molecule. The direction of this discrepancy is unprecedented on the basis of 
      experience with loss of enzymatic activity by irradiation; indeed, two enzymes 
      which were present in the receptor preparations gave expected values when 
      measured simultaneously. We suggest that in instances where a function such as 
      ligand binding resides in a conformationally stable domain, radiation 
      inactivation may be capable of revealing this.
FAU - Fewtrell, C
AU  - Fewtrell C
FAU - Kempner, E
AU  - Kempner E
FAU - Poy, G
AU  - Poy G
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Immunologic)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.2.1.31 (Glucuronidase)
RN  - EC 3.2.1.52 (Acetylglucosaminidase)
SB  - IM
MH  - Acetylglucosaminidase/metabolism
MH  - Animals
MH  - Basophils/immunology
MH  - Cell Membrane/immunology
MH  - Glucuronidase/metabolism
MH  - Immunoglobulin E/*metabolism
MH  - Kinetics
MH  - Leukemia, Experimental/enzymology/*immunology
MH  - Molecular Weight
MH  - Rats
MH  - Receptors, IgE
MH  - Receptors, Immunologic/*metabolism/radiation effects
EDAT- 1981/11/10 00:00
MHDA- 1981/11/10 00:01
CRDT- 1981/11/10 00:00
PHST- 1981/11/10 00:00 [pubmed]
PHST- 1981/11/10 00:01 [medline]
PHST- 1981/11/10 00:00 [entrez]
AID - 10.1021/bi00526a011 [doi]
PST - ppublish
SO  - Biochemistry. 1981 Nov 10;20(23):6589-94. doi: 10.1021/bi00526a011.