PMID- 646287
OWN - NLM
STAT- MEDLINE
DCOM- 19780612
LR  - 20170214
IS  - 0003-4894 (Print)
IS  - 0003-4894 (Linking)
VI  - 87
IP  - 2 Pt 1
DP  - 1978 Mar-Apr
TI  - Immunoglobulin E in chronic middle ear effusions: conmparison of RIST, PRIST, and 
      RIA techniques.
PG  - 197-201
AB  - To investigate the possible role of allergy in otitis media with effusion (OME), 
      the immunoglobulin E (IgE) content of 138 middle ear effusions (MEE) and paired 
      serum samples from patients with chronic otitis media with effusion was 
      determined. The initial 62 paired specimens were assayed for IgE by the 
      radioimmunosorbent test (RIST), while the later 76 paired specimens were assayed 
      for IgE by the paper radioimmunosorbent test (PRIST). When the results obtained 
      by these two techniques were compared, it was noted that the PRIST procedure gave 
      significantly lower IgE values for effusions than the RIST method. When the 
      effusion-to-serum ratios (E/S ratios) were computed from the PRIST data, the E/S 
      ratio was less than one, while RIST data gave an E/S ratio greater than one. The 
      results obtained with the PRIST procedure were confirmed by double antibody 
      radioimmunoassay for IgE. Thus, the PRIST procedure appears to measure the IgE 
      content of MEE more accurately, and the results obtained by this procedure fail 
      to support the concept of allergy as a major causative factor in OME.
FAU - Lewis, D M
AU  - Lewis DM
FAU - Schram, J L
AU  - Schram JL
FAU - Lim, D J
AU  - Lim DJ
FAU - Birck, H G
AU  - Birck HG
FAU - Gleich, G
AU  - Gleich G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Ann Otol Rhinol Laryngol
JT  - The Annals of otology, rhinology, and laryngology
JID - 0407300
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Chronic Disease
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Infant
MH  - Otitis Media/*immunology
MH  - Radioimmunoassay
MH  - Radioimmunosorbent Test
EDAT- 1978/03/01 00:00
MHDA- 1978/03/01 00:01
CRDT- 1978/03/01 00:00
PHST- 1978/03/01 00:00 [pubmed]
PHST- 1978/03/01 00:01 [medline]
PHST- 1978/03/01 00:00 [entrez]
AID - 10.1177/000348947808700209 [doi]
PST - ppublish
SO  - Ann Otol Rhinol Laryngol. 1978 Mar-Apr;87(2 Pt 1):197-201. doi: 
      10.1177/000348947808700209.