PMID- 6472270
OWN - NLM
STAT- MEDLINE
DCOM- 19841003
LR  - 20131121
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 18
IP  - 3
DP  - 1984 May-Jun
TI  - [Protein fluorescence of photosynthetic reaction centers from Rhodopseudomonas 
      sphaeroides].
PG  - 719-24
AB  - Luminescence emitted by tryptophan residues of reaction center (RC) preparations 
      was studied. The RG preparations were isolated from the photosynthetic bacterium 
      Rhodopseudomonas sphaeroides by treatment with lauryl dimethyl amine oxide 
      (LDAO). After excitation at lambda 280 nm the quantum yield of luminescence is 
      0,02. It is shown that 60% of tryptophanyls are located inside the protein 
      globule in the surrounding of relaxating polar groups and the rest approximately 
      40% on the outer surface of the globule--predominantly in the positively charged 
      region of the LDAO-RC protein--in the surrounding of protein-bound water 
      molecules. There is a correlation between the pH dependencies of the position of 
      the peak of luminescence from tryptophanyls and effectivity of electron transfer 
      from the primary (quinone) to secondary acceptor. The two parameters are 
      invariant at pH from 7 to 9 and vary at pH less than 7 and pH greater than 9. The 
      phenomena responsible for the observed correlation are discussed on the basis of 
      pH-dependent changes in the RC protein which govern electron transport activity 
      at the reaction center.
FAU - Zakharova, N I
AU  - Zakharova NI
FAU - Permiakov, E A
AU  - Permiakov EA
FAU - Fabian, M
AU  - Fabian M
FAU - Kononenko, A A
AU  - Kononenko AA
FAU - Chamorovskii, S K
AU  - Chamorovskii SK
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - O belkovoi fluorestsentsii fotosinteticheskikh reaktsionnykh tsentrov iz 
      Rhodopseudomonas sphaeroides.
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (Bacterial Proteins)
RN  - 0 (Detergents)
RN  - 0 (Dimethylamines)
RN  - 0 (Luminescent Proteins)
RN  - 4F6FC4MI8W (dodecyldimethylamine oxide)
RN  - 8DUH1N11BX (Tryptophan)
SB  - IM
MH  - Bacterial Chromatophores/*metabolism
MH  - Bacterial Proteins/isolation & purification/*metabolism
MH  - Binding Sites
MH  - Detergents
MH  - Dimethylamines
MH  - Electron Transport
MH  - Hydrogen-Ion Concentration
MH  - Luminescent Measurements
MH  - Luminescent Proteins/isolation & purification/*metabolism
MH  - Rhodopseudomonas/*metabolism
MH  - Spectrophotometry, Ultraviolet
MH  - Tryptophan/metabolism
EDAT- 1984/05/01 00:00
MHDA- 1984/05/01 00:01
CRDT- 1984/05/01 00:00
PHST- 1984/05/01 00:00 [pubmed]
PHST- 1984/05/01 00:01 [medline]
PHST- 1984/05/01 00:00 [entrez]
PST - ppublish
SO  - Mol Biol (Mosk). 1984 May-Jun;18(3):719-24.