PMID- 6504065
OWN - NLM
STAT- MEDLINE
DCOM- 19841231
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 129
IP  - 2
DP  - 1984 Nov
TI  - The nature of mutants induced by ionising radiation in cultured hamster cells. I. 
      Isolation and initial characterisation of spontaneous, ionising 
      radiation-induced, and ethyl methanesulphonate-induced mutants resistant to 
      6-thioguanine.
PG  - 269-81
AB  - A large number of thioguanine (TG)-resistant mutants of V79-4 Chinese hamster 
      cells was isolated from untreated cultures and from cultures exposed to 
      gamma-rays, alpha particles or ethyl methanesulphonate (EMS). Selection 
      conditions were chosen to optimise the survival of all types of TG-resistant 
      mutant, and the isolation procedure ensured that each mutant originated 
      independently of any other. Hypoxanthine-guanine phosphoribosyl transferase 
      (HGPRT) enzyme activity was measured in cell-free extracts of each mutant, and 
      compared with repeat measurements made on the parental V79-4 cells and on a 
      series of non-mutant clones. Ionising radiation-induced mutants were found to be 
      mostly (or perhaps entirely) of the 'zero HGPRT activity' type, but about 20% of 
      EMS-induced mutants and 50% of spontaneously occurring mutants had significant 
      HGPRT activity. However, none of the TG-resistant mutants were found to lack 
      activity of another X-chromosome-linked enzyme, glucose-6-phosphate 
      dehydrogenase. Few mutants were found with visible X-chromosome changes, but the 
      incidence of hyperploidy was higher among spontaneous mutants than in the 
      parental line and the induced mutants. Isoelectric focussing of cell extracts 
      from those mutants which retained some HGPRT activity revealed several with 
      shifts in the isoelectric points for HGPRT enzyme activity.
FAU - Brown, R
AU  - Brown R
FAU - Thacker, J
AU  - Thacker J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 9H154DI0UP (Ethyl Methanesulfonate)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
RN  - FTK8U1GZNX (Thioguanine)
SB  - IM
MH  - Animals
MH  - Cricetinae
MH  - Cricetulus
MH  - Drug Resistance/drug effects/radiation effects
MH  - Ethyl Methanesulfonate/toxicity
MH  - Gamma Rays
MH  - Glucosephosphate Dehydrogenase/genetics
MH  - Hypoxanthine Phosphoribosyltransferase/genetics
MH  - Karyotyping
MH  - Male
MH  - Mutation/*radiation effects
MH  - Radiation, Ionizing
MH  - Thioguanine
EDAT- 1984/11/01 00:00
MHDA- 1984/11/01 00:01
CRDT- 1984/11/01 00:00
PHST- 1984/11/01 00:00 [pubmed]
PHST- 1984/11/01 00:01 [medline]
PHST- 1984/11/01 00:00 [entrez]
AID - 0027-5107(84)90160-X [pii]
AID - 10.1016/0027-5107(84)90160-x [doi]
PST - ppublish
SO  - Mutat Res. 1984 Nov;129(2):269-81. doi: 10.1016/0027-5107(84)90160-x.