PMID- 6504066
OWN - NLM
STAT- MEDLINE
DCOM- 19850117
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 134
IP  - 2-3
DP  - 1984 Sep-Nov
TI  - A report of the U.S. Environmental Protection Agency Gene-Tox Program. Evaluation 
      of mutagenicity assays for purposes of genetic risk assessment.
PG  - 143-57
AB  - For the vast majority of chemicals, mammalian germ-line (MG) mutation data do not 
      exist. The question was examined of how best to utilize results of non-MG 
      genotoxicity assays that are included in the Gene-Tox data base to provide 
      information of the likelihood that genetic damage might be induced in and 
      transmitted by the reproductive cells of exposed human beings. Two approaches 
      were used to assess the relative value of different assays for genetic hazard 
      identification. (1) Test results were weighted according to parameters by which 
      conditions of an assay resemble those encountered in the potential induction of 
      transmitted genetic damage in mammals. For this purpose, 35 assays were grouped 
      into 16 categories that were assigned weights ranging from 1 to 15; there were 
      2367 chemicals in the data base. This system was evaluated by comparing the sum 
      of weighted test results for each chemical with the outcome of MG-standard (MGst) 
      tests where such had been reported. (MGst tests used were the specific-locus and 
      heritable-translocation assays [SLT and HTT] for gene mutations and chromosome 
      aberrations, respectively.) The weighting system produced a few false positives 
      with respect to the MGst results. It produced no false negatives, but the 
      available evidence is limited by the circumstance that MGst test have evidently 
      been preferentially performed with chemicals that had already been shown to be 
      positive in several other assays. (2) Findings from each MGst test were compared 
      with those from each of the other assays in turn, provided that at least 10 
      chemicals had been tested in both of the assays. There were 11 such comparisons 
      involving the SLT, and 14 such comparisons involving the HTT. The observed 
      concordance was above random expectation in several comparisons, particularly 
      those involving certain mammalian in vivo tests, but in only one case (HTT vs. 
      unscheduled DNA synthesis in the testis) did the degree of elevation approach 
      statistical significance.
FAU - Russell, L B
AU  - Russell LB
FAU - Aaron, C S
AU  - Aaron CS
FAU - de Serres, F
AU  - de Serres F
FAU - Generoso, W M
AU  - Generoso WM
FAU - Kannan, K L
AU  - Kannan KL
FAU - Shelby, M
AU  - Shelby M
FAU - Springer, J
AU  - Springer J
FAU - Voytek, P
AU  - Voytek P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
SB  - IM
MH  - Animals
MH  - Chromosome Aberrations
MH  - Cricetinae
MH  - Female
MH  - Genes/*drug effects
MH  - Humans
MH  - Male
MH  - Mutagenicity Tests
MH  - Mutagens/*toxicity
MH  - *Mutation
MH  - Risk
MH  - Sister Chromatid Exchange/drug effects
MH  - Species Specificity
MH  - United States
MH  - United States Environmental Protection Agency
EDAT- 1984/09/01 00:00
MHDA- 1984/09/01 00:01
CRDT- 1984/09/01 00:00
PHST- 1984/09/01 00:00 [pubmed]
PHST- 1984/09/01 00:01 [medline]
PHST- 1984/09/01 00:00 [entrez]
AID - 0165-1110(84)90008-3 [pii]
AID - 10.1016/0165-1110(84)90008-3 [doi]
PST - ppublish
SO  - Mutat Res. 1984 Sep-Nov;134(2-3):143-57. doi: 10.1016/0165-1110(84)90008-3.