PMID- 6507086
OWN - NLM
STAT- MEDLINE
DCOM- 19850124
LR  - 20190904
IS  - 0001-6632 (Print)
IS  - 0001-6632 (Linking)
VI  - 34
IP  - 5
DP  - 1984 Sep
TI  - Electron microscopic localization of acridine orange binding to DNA within 
      various human brain tumor cells.
PG  - 1041-7
AB  - The ultracytochemical acridine orange (AO) method has been employed to 
      demonstrate DNA template activity within untreated human brain tumors. A total of 
      16 biopsies from brain tumors removed for frozen section examination were 
      investigated. Ultrastructural examination revealed that AO binds to DNA 
      exclusively within the extended euchromatin portion of the cell nucleus of brain 
      tumors. The percentage of AO positive cells varied from 6.0 to 48.6% in various 
      types of brain tumors. A few AO reaction products were visible in the nuclei of 
      the endothelial cells and in the pericytes of the capillaries within neoplastic 
      tissue. The present results suggested that untreated human brain tumors exhibit 
      de-repression of DNA template normally repressed in the adult state. This altered 
      DNA templates may be related to abnormal cell proliferation and differentiation 
      diverted from the normal state.
FAU - Hara, H
AU  - Hara H
FAU - Moriki, T
AU  - Moriki T
FAU - Matsusaki, K
AU  - Matsusaki K
FAU - Hiroi, M
AU  - Hiroi M
FAU - Yamane, T
AU  - Yamane T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Acta Pathol Jpn
JT  - Acta pathologica japonica
JID - 0372637
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*metabolism
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biopsy
MH  - Brain Neoplasms/*pathology/ultrastructure
MH  - DNA/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Templates, Genetic
EDAT- 1984/09/01 00:00
MHDA- 1984/09/01 00:01
CRDT- 1984/09/01 00:00
PHST- 1984/09/01 00:00 [pubmed]
PHST- 1984/09/01 00:01 [medline]
PHST- 1984/09/01 00:00 [entrez]
AID - 10.1111/j.1440-1827.1984.tb07634.x [doi]
PST - ppublish
SO  - Acta Pathol Jpn. 1984 Sep;34(5):1041-7. doi: 10.1111/j.1440-1827.1984.tb07634.x.