PMID- 6507098
OWN - NLM
STAT- MEDLINE
DCOM- 19850117
LR  - 20191031
IS  - 0108-0164 (Print)
IS  - 0108-0164 (Linking)
VI  - 92
IP  - 5
DP  - 1984 Sep
TI  - Cytofluorometric quantitation of acridine orange uptake by cultured cells.
PG  - 303-9
AB  - The vacuolar accumulation of the lysosomotropic weak base acridine orange (AO) 
      within living cells in culture was studied by cytofluorometry. Mouse peritoneal 
      macrophages, malignant human glioma cells, and normal human glial cells were 
      utilized. Exposure to AO resulted in granular bright red fluorescence, as well as 
      a diffuse weak green background fluorescence. To obtain reproducible "staining" 
      conditions, the red granular fluorescence was measured as a function of dye 
      concentration and staining time. Exposure to high concentrations of AO (greater 
      than 10 micrograms/ml) was found to cause cell damage in combination with 
      markedly changed fluorescence distribution for the cell population with reduced 
      mean fluorescence and increased variability. Granular uptake of AO was 
      pH-dependent and almost zero at pH 5.5. AO fluorescence, as measured by 
      cytofluorometry, was found to be roughly linear to the amount of AO present in 
      the cells, as measured by spectrofluorometry after cell solubilization, 
      indicating negligible fluorescence quenching. AO labelling of living cells might 
      serve as a useful indicator of the condition of the cellular vacuolar (lysosomal) 
      apparatus.
FAU - Rundquist, I
AU  - Rundquist I
FAU - Olsson, M
AU  - Olsson M
FAU - Brunk, U
AU  - Brunk U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Acta Pathol Microbiol Immunol Scand A
JT  - Acta pathologica, microbiologica, et immunologica Scandinavica. Section A, 
      Pathology
JID - 8206622
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*metabolism
MH  - Animals
MH  - *Biological Transport
MH  - Cells, Cultured
MH  - Flow Cytometry/methods
MH  - Glioma/*metabolism/pathology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Macrophages/cytology/*metabolism
MH  - Mice
MH  - Neuroglia/cytology/*metabolism
EDAT- 1984/09/01 00:00
MHDA- 1984/09/01 00:01
CRDT- 1984/09/01 00:00
PHST- 1984/09/01 00:00 [pubmed]
PHST- 1984/09/01 00:01 [medline]
PHST- 1984/09/01 00:00 [entrez]
AID - 10.1111/j.1699-0463.1984.tb04408.x [doi]
PST - ppublish
SO  - Acta Pathol Microbiol Immunol Scand A. 1984 Sep;92(5):303-9. doi: 
      10.1111/j.1699-0463.1984.tb04408.x.