PMID- 6507619
OWN - NLM
STAT- MEDLINE
DCOM- 19850124
LR  - 20181130
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 247
IP  - 6 Pt 1
DP  - 1984 Dec
TI  - Epithelial response to intestinal anaphylaxis in rats: goblet cell secretion and 
      enterocyte damage.
PG  - G632-7
AB  - The effects of immunoglobulin E (IgE)-mediated reactions on the intestinal 
      epithelium were examined during intestinal anaphylaxis in the rat. Rats 
      sensitized by intraperitoneal injection of egg albumin (EA) plus alum developed 
      high serum titers of IgE anti-EA antibodies after 14 days; sham-treated 
      littermate controls had no anti-EA antibodies. Two isolated loops of jejunum were 
      prepared in vivo in anesthetized rats. The loops were injected with EA in saline 
      or saline alone, and intraluminal contents of each loop were examined after 4 h. 
      Mucosal histamine decreased in sensitized rat intestine exposed to EA. Luminal 
      mucin, measured by radioimmunoassay, was not increased by antigen challenge. In 
      contrast, DNA, protein, and sucrase activities were elevated in contents from the 
      isolated segments exposed to EA in sensitized rats. Histology revealed that 
      periodic acid-Schiff-stained material was contained in goblet cells in sections 
      prepared from these segments after antigen exposure. Cellular debris was present 
      over the tips of the villi. These findings suggest that IgE-mediated reactions in 
      the intestine cause epithelial damage and loss of material from cells other than 
      goblet cells. The results indicate that release of goblet cell mucus is not a 
      feature of intestinal anaphylaxis.
FAU - Perdue, M H
AU  - Perdue MH
FAU - Forstner, J F
AU  - Forstner JF
FAU - Roomi, N W
AU  - Roomi NW
FAU - Gall, D G
AU  - Gall DG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Albumins)
RN  - 0 (Mucins)
SB  - IM
MH  - Albumins/immunology
MH  - Anaphylaxis/*metabolism/pathology
MH  - Animals
MH  - Epithelium/metabolism/pathology
MH  - Intestinal Diseases/*metabolism/pathology
MH  - Intestinal Mucosa/*metabolism
MH  - Intestines/pathology
MH  - Mucins/metabolism
MH  - Periodic Acid-Schiff Reaction
MH  - Radioimmunoassay
MH  - Rats
EDAT- 1984/12/01 00:00
MHDA- 1984/12/01 00:01
CRDT- 1984/12/01 00:00
PHST- 1984/12/01 00:00 [pubmed]
PHST- 1984/12/01 00:01 [medline]
PHST- 1984/12/01 00:00 [entrez]
AID - 10.1152/ajpgi.1984.247.6.G632 [doi]
PST - ppublish
SO  - Am J Physiol. 1984 Dec;247(6 Pt 1):G632-7. doi: 10.1152/ajpgi.1984.247.6.G632.