PMID- 6508212
OWN - NLM
STAT- MEDLINE
DCOM- 19850114
LR  - 20170214
IS  - 0004-5632 (Print)
IS  - 0004-5632 (Linking)
VI  - 21 ( Pt 5)
DP  - 1984 Sep
TI  - Performance of three enzymic methods for filter paper glucose determination.
PG  - 411-6
AB  - Collection of blood spots on filter paper offers a practical alternative for home 
      monitoring of diabetic patients. We have compared the merits of three protein 
      precipitants, trichloracetic acid (TCA), perchloric acid (PCA) and 
      sulphosalicylic acid (SSA) for the elution of glucose from the filter paper, and 
      their subsequent effects on three enzymic methods, glucose dehydrogenase (GDH), 
      hexokinase (HK), and glucose oxidase (GOD) for the determination of glucose using 
      a microcentrifugal analyser. The combination of TCA elutant with the GDH method 
      was superior with respect to time course of reaction and elution time from the 
      filter paper, and was chosen for routine use. Within- and between-batch precision 
      for this method was 2.7% and 3.2% respectively at normal glucose concentrations. 
      Recovery of glucose added to whole blood was 110 +/- 5%. Comparison with an 
      automated glucose oxidase method for plasma glucose gave a slope of 1.1, 
      intercept of -0.7 and a correlation coefficient of 0.9 (n = 64). We conclude that 
      the combination of TCA and glucose dehydrogenase provides a robust, precise and 
      accurate method for the quantitation of glucose in filter-paper blood spots. The 
      procedure offers increased sensitivity and better precision than GOD methods. The 
      use of TCA as elutant gives a faster elution time and has the least effect on any 
      of the enzymic methods.
FAU - Burrin, J M
AU  - Burrin JM
FAU - Price, C P
AU  - Price CP
LA  - eng
PT  - Journal Article
PL  - England
TA  - Ann Clin Biochem
JT  - Annals of clinical biochemistry
JID - 0324055
RN  - 0 (Blood Glucose)
RN  - EC 1.1.1.- (Glucose Dehydrogenases)
RN  - EC 1.1.1.47 (Glucose 1-Dehydrogenase)
RN  - EC 1.1.3.4 (Glucose Oxidase)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Blood Glucose/*analysis
MH  - Diabetes Mellitus/blood
MH  - Glucose 1-Dehydrogenase
MH  - Glucose Dehydrogenases/metabolism
MH  - Glucose Oxidase/metabolism
MH  - Hexokinase/metabolism
MH  - Humans
MH  - Paper
MH  - Self Care/*methods
MH  - Time Factors
EDAT- 1984/09/01 00:00
MHDA- 1984/09/01 00:01
CRDT- 1984/09/01 00:00
PHST- 1984/09/01 00:00 [pubmed]
PHST- 1984/09/01 00:01 [medline]
PHST- 1984/09/01 00:00 [entrez]
AID - 10.1177/000456328402100513 [doi]
PST - ppublish
SO  - Ann Clin Biochem. 1984 Sep;21 ( Pt 5):411-6. doi: 10.1177/000456328402100513.