PMID- 6515716
OWN - NLM
STAT- MEDLINE
DCOM- 19850131
LR  - 20141120
IS  - 0041-3771 (Print)
IS  - 0041-3771 (Linking)
VI  - 26
IP  - 10
DP  - 1984 Oct
TI  - [Changes in hexokinase activity during muscle alteration].
PG  - 1145-50
AB  - Changes in extractability and activity of hexokinase (HK) were studied under the 
      action of heating and of urea on skeletal muscles of Rana temporaria L., and 
      besides the stability of this enzyme in muscle extract to those agents in vitro 
      was examined. Under a 15 minutes heating of muscle, a decrease in extractability 
      (the activity calculated for 1 g of tissue) and activity (the activity calculated 
      for 1 mg of protein) of hexokinase is first revealed at 37 degrees C. Then the 
      enzyme extractability decreases gradually in accordance to the decrease in 
      extractability of the total water-soluble protein; the level of hexokinase 
      activity attained at 37 degrees does not change up to 40 degrees. At 42 degrees 
      the activity of the enzyme is completely inhibited. Under the heating of the 
      muscle extract, the decrease of enzyme activity takes place at 36 degrees, the 
      level achieved being stable up to 42 degrees C. Under the action of urea on the 
      muscle at the reversible phase of alteration (1 M urea from 5 minutes to 2 hours 
      at room temperature, 1 M urea for 9 hours at + 4 degrees C), hexokinase activity 
      increases, calculated for 1 g of tissue and for 1 mg of protein. Under the 
      irreversible disappearance of muscle excitability (1 M urea during 9 hours, 2 M 
      urea during 2 hours at room temperature) no hexokinase activity was revealed. The 
      activation of the enzyme is discussed in connection with the data on the increase 
      of ATP content in muscle under the urea alteration. The treatment of the enzyme 
      in muscle extract with 1 M urea decreases its activity in 30 minutes down to 67%; 
      the level achieved does not change during 20 hours.
FAU - Stabrovskaia, V I
AU  - Stabrovskaia VI
FAU - Krauzova, N F
AU  - Krauzova NF
FAU - Braun, A D
AU  - Braun AD
LA  - rus
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
TT  - Izmeneniia aktivnosti geksokinazy pri al'teratsii myshtsy.
PL  - Russia (Federation)
TA  - Tsitologiia
JT  - Tsitologiia
JID - 0417363
RN  - 8W8T17847W (Urea)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Aerobiosis
MH  - Animals
MH  - Glycolysis
MH  - Hexokinase/isolation & purification/*metabolism
MH  - Hot Temperature
MH  - In Vitro Techniques
MH  - Muscles/drug effects/*enzymology
MH  - Solubility
MH  - Temperature
MH  - Time Factors
MH  - Urea/pharmacology
EDAT- 1984/10/01 00:00
MHDA- 1984/10/01 00:01
CRDT- 1984/10/01 00:00
PHST- 1984/10/01 00:00 [pubmed]
PHST- 1984/10/01 00:01 [medline]
PHST- 1984/10/01 00:00 [entrez]
PST - ppublish
SO  - Tsitologiia. 1984 Oct;26(10):1145-50.