PMID- 6518936
OWN - NLM
STAT- MEDLINE
DCOM- 19850307
LR  - 20071114
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 5
IP  - 6
DP  - 1984 Nov
TI  - Flow cytometric analysis of blood cells stained with the cyanine dye DiOC1[3]: 
      reticulocyte quantification.
PG  - 589-600
AB  - The fluorescent dye 3,3'-dimethyloxacarbocyanine (DiOC1[3]) is taken up by all 
      cells in mammalian blood which then fluoresce as follows: mature erythrocytes 
      less than immature erythrocytes congruent to platelets less than leukocytes. A 
      continuous fluorescence distribution can be generated for the red blood cells by 
      flow cytometry and deconvolved into two arbitrary populations, mature and 
      immature erythrocytes (mRBC and imRBC). This analysis mimics the established 
      method of counting imRBC stained with the supravital dyes, new methylene blue, 
      brilliant cresyl blue (BCB), and acridine orange (AO). However, the population of 
      imRBC as quantified by DiOC1[3] fluorescence is a subset of reticulocytes 
      (reticulocytes as determined by BCB assay). The advantages and disadvantages of 
      using DiOC1[3], AO, or pyronine Y as reticulocyte stains are discussed.
FAU - Jacobberger, J W
AU  - Jacobberger JW
FAU - Horan, P K
AU  - Horan PK
FAU - Hare, J D
AU  - Hare JD
LA  - eng
GR  - 5 T32 AI 07137/AI/NIAID NIH HHS/United States
GR  - P01 GM 23088/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Animals
MH  - Erythrocyte Aging
MH  - Erythrocyte Count/methods
MH  - Erythrocytes/cytology
MH  - Female
MH  - Flow Cytometry/methods
MH  - Fluorescent Dyes
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Reticulocytes/*cytology
EDAT- 1984/11/01 00:00
MHDA- 1984/11/01 00:01
CRDT- 1984/11/01 00:00
PHST- 1984/11/01 00:00 [pubmed]
PHST- 1984/11/01 00:01 [medline]
PHST- 1984/11/01 00:00 [entrez]
AID - 10.1002/cyto.990050607 [doi]
PST - ppublish
SO  - Cytometry. 1984 Nov;5(6):589-600. doi: 10.1002/cyto.990050607.