PMID- 65216
OWN - NLM
STAT- MEDLINE
DCOM- 19770415
LR  - 20061115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 37
IP  - 3
DP  - 1977 Mar
TI  - The influence of antisera specific for alpha-fetoprotein and mouse serum albumin 
      on the viability and protein synthesis of cultured mouse hepatoma cells.
PG  - 696-701
AB  - The cytotoxic potential of heterologous rabbit antibody directed against mouse 
      serum albumin (MSA) and alpha-fetoprotein (AFP) was investigated in vitro with a 
      cell line (Hepa) derived from the mouse hepatoma BW7756. Anti-AFP in the presence 
      of complement could kill Hepa cells at concentrations of anti-MSA that were 
      virtually nontoxic. The specificity of the anti-AFP was defined by demonstrating 
      that Hepa cell toxicity was dependent upon and paralleled the secretion of AFP in 
      synchronized cultures. Furthermore, neither antiserum could be shown to be 
      significantly toxic to mouse neuroblastoma cells (Neuro-2A). Immunoglobulin 
      purified from pools of antisera was also highly effective in producing 
      cytotoxicity even in a complement-free system. This reaction proceeded more 
      slowly, requiring nearly 48 hr to reach maximum effect in comparison to the 12 hr 
      for complement-mediated toxicity. MSA and AFP are secreted during different 
      phases of the cell cycle. In cultures arrested by isoleucine starvation, labeled 
      AFP appears in the medium 10 hr after release of the blockade in association with 
      S phase. The appearance of labeled MSA is delayed until the first mitosis. 
      Cytotoxic effects of anti-AFP parallel the secretion of AFP in synchronous 
      cultures. Both antisera could be inhibitory to the secretion and synthesis of the 
      proteins of their antigenic specificity. MSA synthesis was more susceptible to 
      this inhibition than was AFP synthesis. The significance of this phenomenon and 
      its association with the differential cytotoxicity of the antiserum are 
      discussed.
FAU - Allen, R P
AU  - Allen RP
FAU - Ledford, B E
AU  - Ledford BE
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Antibodies)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Serum Albumin)
RN  - 0 (alpha-Fetoproteins)
SB  - IM
MH  - Antibodies
MH  - Antibody Specificity
MH  - Carcinoma, Hepatocellular/*immunology/metabolism
MH  - Cell Division
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Cytotoxicity Tests, Immunologic
MH  - Liver Neoplasms/*immunology/metabolism
MH  - Neoplasm Proteins/biosynthesis
MH  - Neoplasms, Experimental/immunology/metabolism
MH  - Serum Albumin/biosynthesis/*immunology
MH  - alpha-Fetoproteins/biosynthesis/*immunology
EDAT- 1977/03/01 00:00
MHDA- 1977/03/01 00:01
CRDT- 1977/03/01 00:00
PHST- 1977/03/01 00:00 [pubmed]
PHST- 1977/03/01 00:01 [medline]
PHST- 1977/03/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1977 Mar;37(3):696-701.