PMID- 6521739
OWN - NLM
STAT- MEDLINE
DCOM- 19850314
LR  - 20061115
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 18
IP  - 6
DP  - 1984 Nov-Dec
TI  - [Study of the structural organization of RNA from phage MS2 using fluorescent 
      dyes].
PG  - 1617-24
AB  - The spatial organization of phage MS2 RNA by binding to ethidium bromide (EtBr) 
      and acridine orange (AO) to RNA was studied. The analyses of dye interaction by 
      spectrophotometric and fluorometric methods have demonstrated that only about a 
      half of 65-70% nucleotides of double-stranded segments can interact with AO and 
      EtBr. On the other hand all the single-stranded segments appear to be accessible 
      to AO binding. These interactions did not practically change when ionic strength 
      (0.01-0.3), Mg2+ and Zn2+ concentrations (10(-3) M) or pH (4.7-7.4) varied. The 
      data permit to suppose that phage MS2 RNA has a very stable tertiary structure 
      which makes part of double-stranded segments unaccessible to inter calating dyes. 
      Taking these and other facts into consideration we suppose that double-stranded 
      segments play an important role in stabilization of the RNA tertiary structure. 
      One of the most possible structure is a compact "rod-like" intramolecular 
      aggregate of double-stranded hairpin-like segments of RNA with parallel 
      orientation.
FAU - Borisova, O F
AU  - Borisova OF
FAU - Grechko, V V
AU  - Grechko VV
FAU - Aleshkina, L A
AU  - Aleshkina LA
FAU - Kuznetsova, N V
AU  - Kuznetsova NV
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Izuchenie strukturnoi organizatsii RNK faga MS2 s ispol'zovaniem 
      fluorestsiruiushchikh krasitelei.
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - *Coliphages
MH  - *Fluorescent Dyes
MH  - Models, Biological
MH  - *Nucleic Acid Conformation
MH  - RNA, Double-Stranded
MH  - *RNA, Viral
EDAT- 1984/11/01 00:00
MHDA- 1984/11/01 00:01
CRDT- 1984/11/01 00:00
PHST- 1984/11/01 00:00 [pubmed]
PHST- 1984/11/01 00:01 [medline]
PHST- 1984/11/01 00:00 [entrez]
PST - ppublish
SO  - Mol Biol (Mosk). 1984 Nov-Dec;18(6):1617-24.