PMID- 6546764
OWN - NLM
STAT- MEDLINE
DCOM- 19840514
LR  - 20061115
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 58
IP  - 5
DP  - 1984 May
TI  - Expression of the proopiomelanocortin gene in human medullary thyroid carcinoma.
PG  - 904-8
AB  - mRNA isolated from medullary carcinoma of the thyroid (MTC) in six patients with 
      the inherited multiple endocrine neoplasia syndrome type 2 and cervical 
      metastases in two patients with sporadic MTC was screened for the presence of 
      calcitonin and proopiomelanocortin (POMC) related sequences by blot hybridization 
      analysis. All tumors were found to contain mRNA hybridizing to a 
      calcitonin-specific probe. However, POMC-related sequences were detected only in 
      RNA preparations purified from the metastases of MTC. Compared with the POMC mRNA 
      occurring in the human pituitary gland, the size of the RNA species hybridizing 
      to the POMC-specific probe was larger in the metastases of both of the patients 
      investigated. cDNA complementary to mRNA from one of the metastases was cloned 
      into pBR 322. From this library two plasmids were isolated containing inserts 
      which were completely homologous to a sequence of 190 nucleotides from the 3'-end 
      of human pituitary POMC mRNA. In conclusion, 1) hybridization and nucleotide 
      sequence analyses demonstrate that the POMC gene is expressed in MTC metastases; 
      2) the observed length difference between MTC and pituitary mRNAs suggests 
      differences in expression or processing; 3) no expression of the POMC gene was 
      found in six primary tumors.
FAU - Steenbergh, P H
AU  - Steenbergh PH
FAU - Hoppener, J W
AU  - Hoppener JW
FAU - Zandberg, J
AU  - Zandberg J
FAU - Roos, B A
AU  - Roos BA
FAU - Jansz, H S
AU  - Jansz HS
FAU - Lips, C J
AU  - Lips CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Pituitary Hormones, Anterior)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 66796-54-1 (Pro-Opiomelanocortin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - Carcinoma/genetics/*metabolism
MH  - DNA/metabolism
MH  - DNA, Neoplasm/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Hybridization
MH  - Pituitary Hormones, Anterior/biosynthesis/*genetics
MH  - Pro-Opiomelanocortin
MH  - Protein Precursors/biosynthesis/*genetics
MH  - RNA, Messenger/metabolism
MH  - RNA, Neoplasm/metabolism
MH  - Thyroid Neoplasms/genetics/*metabolism
EDAT- 1984/05/01 00:00
MHDA- 1984/05/01 00:01
CRDT- 1984/05/01 00:00
PHST- 1984/05/01 00:00 [pubmed]
PHST- 1984/05/01 00:01 [medline]
PHST- 1984/05/01 00:00 [entrez]
AID - 10.1210/jcem-58-5-904 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1984 May;58(5):904-8. doi: 10.1210/jcem-58-5-904.