PMID- 656502
OWN - NLM
STAT- MEDLINE
DCOM- 19780814
LR  - 20191210
IS  - 0320-9725 (Print)
IS  - 0320-9725 (Linking)
VI  - 43
IP  - 5
DP  - 1978 May
TI  - [Purification and properties of 3-hexulosephosphate synthase from facultative 
      methylotroph Pseudomonas oleovorans].
PG  - 782-8
AB  - 3-Hexulosephosphate synthase (HPS), the key enzyme of the hexulosephosphate cycle 
      of formaldehyde fixation, was isolated from facultative methylotroph Pseudomonas 
      oleovorans. Enzyme was purified 100-fold. The purification procedure involved 
      fractionation with ammonium sulfate, gel-filtration on Sephadex G-150 and 
      chromatography on DEAE-Sephadex A-50. The purified enzyme gave single band on 
      analytical polyacrylamide gel electrophoresis. Optimal conditions for activity of 
      HPS are: pH 7,0, temperature 50 degrees C. The molecular weight was calculated to 
      be 45 000 from gel-filtration experiments. HPS is active only in the presence of 
      Mg2+ or Mn2+. Ribulose-5-phosphate is the sole acceptor of formaldehyde. Activity 
      of the enzyme is inhibited by NADH and NADPH.
FAU - Sokolov, A P
AU  - Sokolov AP
FAU - Trotsenko, Y A
AU  - Trotsenko YA
LA  - rus
PT  - Journal Article
TT  - Ochistka i kharakteristika 3-geksulozofosfatsintazy iz fakul'tativnogo 
      metilotrofa Pseudomonas oleovorans.
PL  - Russia (Federation)
TA  - Biokhimiia
JT  - Biokhimiia (Moscow, Russia)
JID - 0372667
RN  - 0 (Hexosephosphates)
RN  - 0 (Ribulosephosphates)
RN  - EC 4.1.2.- (Aldehyde-Lyases)
RN  - EC 4.1.2.- (hexose phosphate synthetase)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Aldehyde-Lyases/isolation & purification/*metabolism
MH  - Hexosephosphates
MH  - Kinetics
MH  - Methane/metabolism
MH  - Molecular Weight
MH  - Pseudomonas/*enzymology
MH  - Ribulosephosphates
EDAT- 1978/05/01 00:00
MHDA- 1978/05/01 00:01
CRDT- 1978/05/01 00:00
PHST- 1978/05/01 00:00 [pubmed]
PHST- 1978/05/01 00:01 [medline]
PHST- 1978/05/01 00:00 [entrez]
PST - ppublish
SO  - Biokhimiia. 1978 May;43(5):782-8.