PMID- 6569873
OWN - NLM
STAT- MEDLINE
DCOM- 19850306
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 20
IP  - 6
DP  - 1984 Dec
TI  - Detection of methicillin-resistant Staphylococcus aureus by microdilution and 
      disk elution susceptibility systems.
PG  - 1068-75
AB  - To determine whether methicillin-resistant (MR) Staphylococcus aureus from 
      different geographic areas are detected reliably by various commercially 
      available microdilution broth and disk elution systems, 73 such isolates obtained 
      from hospitals in 13 cities were tested by a reference method (agar dilution) and 
      by the Microscan, API 3600S, Autobac I, and MS-2 systems. Both Eugonic broth and 
      Low Thymidine Eugonic broth were used in the evaluation of the Autobac I, and two 
      versions of the MS-2 were used. The proportions of isolates categorized as MR by 
      the various methods were: agar dilution method, 99%; Microscan, 100% (if the 
      suggested cut-off of the manufacturer was used); API 3600S, 96%; Autobac I, 84 to 
      93%; and MS-2, 54 to 68%. With the MS-2 system, isolates from Jackson, Miss., 
      were classified as susceptible to methicillin more often than were strains from 
      other cities. With the Autobac I (Eugonic broth), only 55% of isolates from 
      Houston, Tex., were classified as MR, whereas 89% of isolates from all other 
      cities were correctly classified as MR. With the API 3600S, strains from some 
      cities were categorized as nafcillin susceptible, whereas strains from other 
      cities were classified as resistant to nafcillin. The results of this study 
      suggest that future evaluations of antimicrobial susceptibility testing systems 
      should include MR strains of S. aureus from several geographic areas.
FAU - Boyce, J M
AU  - Boyce JM
FAU - Lytle, L S
AU  - Lytle LS
FAU - Walsh, D A
AU  - Walsh DA
LA  - eng
GR  - 5S07 RR05386/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - Q91FH1328A (Methicillin)
SB  - IM
MH  - Humans
MH  - Methicillin/*pharmacology
MH  - Microbial Sensitivity Tests/*methods
MH  - Penicillin Resistance
MH  - Staphylococcus aureus/drug effects/*isolation & purification
PMC - PMC271519
EDAT- 1984/12/01 00:00
MHDA- 1984/12/01 00:01
PMCR- 1984/12/01
CRDT- 1984/12/01 00:00
PHST- 1984/12/01 00:00 [pubmed]
PHST- 1984/12/01 00:01 [medline]
PHST- 1984/12/01 00:00 [entrez]
PHST- 1984/12/01 00:00 [pmc-release]
AID - 10.1128/jcm.20.6.1068-1075.1984 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1984 Dec;20(6):1068-75. doi: 10.1128/jcm.20.6.1068-1075.1984.