PMID- 6579570
OWN - NLM
STAT- MEDLINE
DCOM- 19831220
LR  - 20191031
IS  - 0262-1746 (Print)
IS  - 0262-1746 (Linking)
VI  - 12
IP  - 1
DP  - 1983 Sep
TI  - Radioimmunoassay of prostaglandins and thromboxane B2 in extracted and 
      unextracted urine and serum using an iodinated ligand.
PG  - 11-20
AB  - A comparison of radioimmunoassay (R.I.A.) for 6-keto PGF 1 alpha, PGE2 and TXB2 
      using 125I-histamine ligands and tritiated tracers showed a 7 to 10 fold increase 
      in sensitivity for the 125I derivative, with a resultant reduction in antisera 
      requirements by 80%. Study of the effect of purification of biological samples by 
      organic extraction and thin layer chromatography showed that for human and rat 
      urine, and urine from perfused rat kidneys, direct R.I.A. of unextracted samples 
      gave substantially higher estimates of PGE2, 6-keto PGF1 alpha and TXB2 than 
      R.I.A. purified material. Indomethacin pre-treatment reduced the levels estimated 
      in both extracted and unextracted samples; the results indicating that the higher 
      estimates obtained by direct assay were due to a combination of PG/TXB2 
      metabolites and non-specific interference. In serum from incubated human blood, 
      estimated 6-keto PGF1 alpha and PGE2 levels were significantly higher by direct 
      R.I.A. than after extraction, whereas TXB2 levels were similar. Thus purification 
      procedures are unnecessary when high serum TXB2 levels are being measured by 
      R.I.A. with relatively specific antisera.
FAU - Mahoney, D P
AU  - Mahoney DP
FAU - Barden, A
AU  - Barden A
FAU - Beilin, L J
AU  - Beilin LJ
FAU - Vandongen, R
AU  - Vandongen R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Scotland
TA  - Prostaglandins Leukot Med
JT  - Prostaglandins, leukotrienes, and medicine
JID - 8206868
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Ligands)
RN  - 0 (Prostaglandins)
RN  - 0 (Radioactive Tracers)
RN  - 0 (Thromboxanes)
RN  - 10028-17-8 (Tritium)
RN  - 54397-85-2 (Thromboxane B2)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Animals
MH  - Cross Reactions
MH  - Indomethacin/pharmacology
MH  - *Iodine Radioisotopes
MH  - Ligands
MH  - Prostaglandins/*analysis
MH  - Radioactive Tracers
MH  - Radioimmunoassay/*methods
MH  - Rats
MH  - Thromboxane B2/*analysis
MH  - Thromboxanes/*analysis
MH  - Tritium
EDAT- 1983/09/01 00:00
MHDA- 1983/09/01 00:01
CRDT- 1983/09/01 00:00
PHST- 1983/09/01 00:00 [pubmed]
PHST- 1983/09/01 00:01 [medline]
PHST- 1983/09/01 00:00 [entrez]
AID - 10.1016/0262-1746(83)90063-x [doi]
PST - ppublish
SO  - Prostaglandins Leukot Med. 1983 Sep;12(1):11-20. doi: 
      10.1016/0262-1746(83)90063-x.