PMID- 6582310
OWN - NLM
STAT- MEDLINE
DCOM- 19840321
LR  - 20071114
IS  - 0027-8874 (Print)
IS  - 0027-8874 (Linking)
VI  - 72
IP  - 2
DP  - 1984 Feb
TI  - Enzyme activities in normal, dysplastic, and cancerous human breast tissues.
PG  - 217-24
AB  - The activities of six different enzymes were compared in 29 normal, 34 
      dysplastic, and 80 cancerous (both primary and metastatic) human breast tissues; 
      in MCF-7 cells; and in primary rat mammary tumors. Benign lesions generally 
      showed enzyme activities similar to those of normal breast tissues. Malignant 
      tumors had significantly increased activities of lactate dehydrogenase (LDH), 
      malate dehydrogenase (MDH), fructose-bisphosphate aldolase, hexokinase (HK), 
      pyruvate kinase (PK), and creatine kinase. Enzyme activity in the malignant tumor 
      was always higher than that in apparently normal or fibrocystic tissue from the 
      same patient. Enzyme activities did not correlate with the levels of estrogen and 
      progesterone receptors. LDH, MDH, and HK were elevated to a similar extent in all 
      the tissues examined. Conversely, PK was elevated to a much greater extent in 
      cancerous tissues, particularly in MCF-7 cells. The elevated activities of these 
      enzymes may have diagnostic potential, especially when tumor tissue and 
      apparently normal tissue from the same patient are compared.
FAU - Balinsky, D
AU  - Balinsky D
FAU - Platz, C E
AU  - Platz CE
FAU - Lewis, J W
AU  - Lewis JW
LA  - eng
GR  - N01CB-84222/CB/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Natl Cancer Inst
JT  - Journal of the National Cancer Institute
JID - 7503089
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.37 (Malate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - EC 2.7.3.2 (Creatine Kinase)
RN  - EC 4.1.2.13 (Fructose-Bisphosphate Aldolase)
SB  - IM
MH  - Adenofibroma/enzymology
MH  - Breast Neoplasms/*enzymology/secondary
MH  - Creatine Kinase/metabolism
MH  - Female
MH  - Fibrocystic Breast Disease/*enzymology
MH  - Fructose-Bisphosphate Aldolase/metabolism
MH  - Hexokinase/metabolism
MH  - Humans
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Malate Dehydrogenase/metabolism
MH  - Pyruvate Kinase/metabolism
EDAT- 1984/02/01 00:00
MHDA- 1984/02/01 00:01
CRDT- 1984/02/01 00:00
PHST- 1984/02/01 00:00 [pubmed]
PHST- 1984/02/01 00:01 [medline]
PHST- 1984/02/01 00:00 [entrez]
PST - ppublish
SO  - J Natl Cancer Inst. 1984 Feb;72(2):217-24.