PMID- 6586300
OWN - NLM
STAT- MEDLINE
DCOM- 19840703
LR  - 20190720
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 86
IP  - 1
DP  - 1984 Jun
TI  - Chemotactic subpopulation of macrophage cell line cells (M1 cells) discerned by 
      three macrophage chemotactic factors from delayed hypersensitivity reaction 
      sites.
PG  - 1-13
AB  - The functional specificity of three types of macrophage chemotactic factors ( 
      MCFs ), -a, -b, and -c, from purified protein derivative of tuberculin 
      (PPD)-induced delayed hypersensitivity reaction (DHR) skin sites on guinea pigs, 
      was analyzed using macrophage cell line cells, M1, established from myeloid 
      leukemia cells of a SL/Am strain mouse. M1 cells yielded two subclones: Mk1 
      cells, which were Ia+ and migrated specifically toward MCF-c; and Mm1 cells, 
      which were Ia- and migrated specifically toward MCF-a and -b. These differences 
      show the heterogeneity of the biologic activities of the MCFs in the presence of 
      cell line cells. M-1 cells, blast cells, when grown in continuous culture, 
      migrated toward none of the MCFs . Upon differentiation to mature macrophage-like 
      cells in conditioned medium (M+1 cells), however, they migrated to MCF-a, -b, and 
      -c and the experimental evidence points to the possibility that M+1 cells are 
      separated into subpopulations on the basis of their chemotactic response. 
      Cytotoxic treatment of M+1 cells with anti-Ia antisera resulted in the specific 
      decrease of activity toward MCF-c. Furthermore, MCF-c attracted Ia-positive M+1 
      cells, while MCF-a and -b attracted Ia-negative M+1 cells. Thus, our results 
      indicate the existence of two migrating subpopulations of M+1 cells with 
      specificities for MCF-a, and -b and for MCF-c, respectively. The data suggest 
      that MCF-c attracts Ia-bearing accessory macrophages and MCF-a and -b attract 
      Ia-negative macrophages in the DHR.
FAU - Honda, M
AU  - Honda M
FAU - Masuda, T
AU  - Masuda T
FAU - Yoshimura, T
AU  - Yoshimura T
FAU - Hayashi, H
AU  - Hayashi H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Chemotactic Factors)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Movement
MH  - Chemotactic Factors/*classification/physiology
MH  - *Chemotaxis
MH  - Guinea Pigs
MH  - Histocompatibility Antigens Class II/analysis
MH  - Hypersensitivity, Delayed/*immunology/pathology
MH  - Macrophages/*immunology
MH  - Male
MH  - Mice
MH  - Species Specificity
EDAT- 1984/06/01 00:00
MHDA- 1984/06/01 00:01
CRDT- 1984/06/01 00:00
PHST- 1984/06/01 00:00 [pubmed]
PHST- 1984/06/01 00:01 [medline]
PHST- 1984/06/01 00:00 [entrez]
AID - 0008-8749(84)90354-X [pii]
AID - 10.1016/0008-8749(84)90354-x [doi]
PST - ppublish
SO  - Cell Immunol. 1984 Jun;86(1):1-13. doi: 10.1016/0008-8749(84)90354-x.