PMID- 6601130
OWN - NLM
STAT- MEDLINE
DCOM- 19830505
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 130
IP  - 4
DP  - 1983 Apr
TI  - Changes in B lineage cell population in liver and spleen of normal neonatal mice.
PG  - 1616-21
AB  - The frequency and characteristics of B lymphocyte lineage cells in neonatal 
      murine liver and spleen were studied during the first 10 days after birth. These 
      were distinguished as B cells with surface IgM (slgM), immediate precursors of B 
      cells (pre-B cells) lacking slgM but containing micron-heavy chains of IgM, and 
      earlier precursors that did not synthesize immunoglobulin but could be detected 
      with monoclonal 14.8 antibody. Experiments were also done to relate these to 
      cells capable of clonal proliferation in mitogen-containing semisolid agar 
      cultures and cells that acquire this function only after preculture in liquid 
      medium. Newborn liver contained large numbers of early precursors as well as 
      pre-B cells, and culture studies revealed that a majority of the colony-forming B 
      cells present at that time were slg-. Adherent accessory cells in newborn liver 
      suspensions facilitated the maturation of these into functional B cells in vitro. 
      At most ages, however, numbers of slg+ B cells detected in that tissue were 
      surprisingly low. Possible explanations for this include a rapid exit of newly 
      formed B cells and their immediate precursors from liver and/or a high rate of 
      abortive lg gene rearrangements during the neonatal period. In contrast, whereas 
      the spleen contained early precursors and pre-B cells at birth, these cells 
      steadily declined in number with age as the numbers of slgM+ B cells increased. 
      Adherent cells in liver but not spleen of immunodeficient CBA/N mice suppressed B 
      lymphocyte formation in semisolid or liquid cultures. These observations document 
      population dynamics in B lineage cells during a critical period of development.
FAU - Jyonouchi, H
AU  - Jyonouchi H
FAU - Kincade, P W
AU  - Kincade PW
LA  - eng
GR  - AI-00558/AI/NIAID NIH HHS/United States
GR  - AI-19884/AI/NIAID NIH HHS/United States
GR  - AI-20069/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Receptors, Antigen, B-Cell)
SB  - IM
MH  - Animals
MH  - *Animals, Newborn
MH  - B-Lymphocytes/classification/*cytology/immunology
MH  - Cell Differentiation
MH  - Clone Cells/cytology/immunology
MH  - Hematopoietic Stem Cells/cytology/immunology
MH  - Liver/*cytology
MH  - Lymphocyte Activation
MH  - Lymphocyte Cooperation
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Mice, Mutant Strains
MH  - Receptors, Antigen, B-Cell/analysis
MH  - Spleen/*cytology
EDAT- 1983/04/01 00:00
MHDA- 1983/04/01 00:01
CRDT- 1983/04/01 00:00
PHST- 1983/04/01 00:00 [pubmed]
PHST- 1983/04/01 00:01 [medline]
PHST- 1983/04/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1983 Apr;130(4):1616-21.