PMID- 6602602
OWN - NLM
STAT- MEDLINE
DCOM- 19830715
LR  - 20151119
IS  - 0003-9985 (Print)
IS  - 0003-9985 (Linking)
VI  - 107
IP  - 7
DP  - 1983 Jul
TI  - Detection of Borrelia in acridine orange-stained blood smears by fluorescence 
      microscopy.
PG  - 384-6
AB  - Tick-borne borreliosis (relapsing fever) can be an important, unsuspected cause 
      of febrile illness. The diagnosis is generally made by identifying Borrelia 
      spirochetes in stained peripheral blood smears. Since Borrelia may be difficult 
      to detect with Romanowsky stains, an alternative method, using acridine orange 
      (AO), was used to screen blood smears. Duplicate blood smears of seven patients 
      were examined with the AO technique and Romanowsky stains. In all seven cases 
      spirochetes were easily identified with the AO-stained smears compared with only 
      five cases with Romanowsky stains. In a double-blind laboratory experiment, six 
      of ten duplicate smears from a single patient with mild spirochetemia were 
      positive by AO, whereas only two of ten were positive by Romanowsky stain. We 
      concluded that the AO stain is simple, rapid and more sensitive than Romanowsky 
      methods for detecting cases of low-level spirochetemia.
FAU - Sciotto, C G
AU  - Sciotto CG
FAU - Lauer, B A
AU  - Lauer BA
FAU - White, W L
AU  - White WL
FAU - Istre, G R
AU  - Istre GR
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Arch Pathol Lab Med
JT  - Archives of pathology & laboratory medicine
JID - 7607091
RN  - 1014KSJ86F (Erythromycin Ethylsuccinate)
RN  - 63937KV33D (Erythromycin)
RN  - F30N4O6XVV (Acridine Orange)
RN  - F8VB5M810T (Tetracycline)
SB  - IM
MH  - Acridine Orange
MH  - Adult
MH  - Blood/*microbiology
MH  - Borrelia/*isolation & purification
MH  - Child
MH  - Child, Preschool
MH  - Erythromycin/analogs & derivatives/therapeutic use
MH  - Erythromycin Ethylsuccinate
MH  - Female
MH  - Humans
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Middle Aged
MH  - Relapsing Fever/drug therapy/*microbiology
MH  - Tetracycline/therapeutic use
EDAT- 1983/07/01 00:00
MHDA- 1983/07/01 00:01
CRDT- 1983/07/01 00:00
PHST- 1983/07/01 00:00 [pubmed]
PHST- 1983/07/01 00:01 [medline]
PHST- 1983/07/01 00:00 [entrez]
PST - ppublish
SO  - Arch Pathol Lab Med. 1983 Jul;107(7):384-6.