PMID- 6621246
OWN - NLM
STAT- MEDLINE
DCOM- 19831123
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 33
IP  - 14
DP  - 1983 Oct 3
TI  - Death in ventricular fibrillation induced by isoproterenol in DOCA-salt 
      pretreated rats preceded by changes in myocardial electrolytes.
PG  - 1353-62
AB  - Serum and tissue content of sodium, potassium, magnesium and calcium was 
      determined in controls and desoxycorticosterone acetate (DOCA)-salt treated rats 
      to determine whether electrolyte changes preceded the development of 
      isoproterenol-induced death in ventricular fibrillation. Control Sprague Dawley, 
      male rats, were injected subcutaneously (s.c.) with either saline (Group A) or 
      actinomycin D (0.1 mg/kg; Group B) once daily for 4 days. Other rats received 20 
      mg of DOCA by implantation, drank normal saline and were injected with either 
      saline (Group C) or actinomycin D (Group D) once daily for 4 days. In the first 
      part of the experiment, it was determined that none of 15 rats from Group C died 
      when challenged with isoproterenol (150 micrograms/kg, s.c.) six days later: 
      however, 13 out of 15 rats from Group D died within 29.1 +/- 15.0 minutes (mean 
      +/- S.D.) from isoproterenol injection. Myocardial sodium was elevated (48.8 +/- 
      3.8 versus 36.3 +/- 1.9) and potassium decreased (60.4 +/- 3.4 versus 70.6 +/- 
      3.3, meq/kg wet weight, mean +/- S.D.) in rats that had succumbed to 
      isoproterenol. In the second part of the experiment serum and tissues were 
      removed from control and DOCA-saline pretreated rats before they died in 
      ventricular fibrillation, 20 minutes after isoproterenol. DOCA-saline pretreated 
      rats were hypernatremic and hypokalemic and exhibited higher sodium and lower 
      potassium in skeletal muscle than control rats. Isoproterenol elicited 
      hypokalemia in all rats, but it only elevated sodium and decreased potassium 
      content in the myocardium of rats of Group D, that were more prone to die in 
      ventricular fibrillation. It is concluded that myocardial electrolyte changes 
      precede the onset of ventricular fibrillation and may be associated with the 
      development of this dysrhythmia.
FAU - Guideri, G
AU  - Guideri G
LA  - eng
GR  - HL 20052/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Electrolytes)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 40GP35YQ49 (Desoxycorticosterone)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Animals
MH  - Dactinomycin/pharmacology
MH  - Death, Sudden/*etiology
MH  - Desoxycorticosterone/*pharmacology
MH  - Electrolytes/*metabolism
MH  - Isoproterenol/*pharmacology
MH  - Male
MH  - Myocardium/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Time Factors
MH  - Ventricular Fibrillation/*chemically induced/metabolism
EDAT- 1983/10/03 00:00
MHDA- 1983/10/03 00:01
CRDT- 1983/10/03 00:00
PHST- 1983/10/03 00:00 [pubmed]
PHST- 1983/10/03 00:01 [medline]
PHST- 1983/10/03 00:00 [entrez]
AID - 0024-3205(83)90818-4 [pii]
AID - 10.1016/0024-3205(83)90818-4 [doi]
PST - ppublish
SO  - Life Sci. 1983 Oct 3;33(14):1353-62. doi: 10.1016/0024-3205(83)90818-4.