PMID- 6643599
OWN - NLM
STAT- MEDLINE
DCOM- 19840107
LR  - 20190629
VI  - 277
DP  - 1983 Oct 14
TI  - Rapid, isocratic separation of purine nucleotides using strong anion-exchange 
      high-performance liquid chromatography.
PG  - 137-44
AB  - A method is presented for the rapid, isocratic separation of purine nucleotides 
      using strong anion-exchange high-performance liquid chromatography at ambient 
      temperature. The last peak of interest guanosine 5'-triphosphate (GTP) is eluted 
      within 30 min and immediate reinjection is possible. All adenine and guanine 
      nucleotides can be assayed with a single injection without the use of a gradient 
      for elution. The procedure is particularly useful for the assay of NTP:AMP 
      phosphotransferase reactions and/or the determination of changes in size of 
      cellular purine nucleotide pools and computation of energy charges. An Altex 
      Ultrasil AX prepacked column was used, and virtually identical results were 
      obtained under similar conditions with the Whatman Partisil-10 SAX column. The 
      eluting solution was 200 mM potassium phosphate, pH 6.85.
FAU - Burnette, B
AU  - Burnette B
FAU - McFarland, C R
AU  - McFarland CR
FAU - Batra, P
AU  - Batra P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr
JT  - Journal of chromatography
JID - 0427043
RN  - 0 (Adenine Nucleotides)
RN  - 0 (Anion Exchange Resins)
RN  - 0 (Purine Nucleotides)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adenine Nucleotides/metabolism
MH  - Aerobiosis
MH  - Anaerobiosis
MH  - Animals
MH  - Anion Exchange Resins
MH  - Cells, Cultured
MH  - Chromatography, High Pressure Liquid/methods
MH  - Energy Metabolism
MH  - Glucose/metabolism
MH  - Myocardium/metabolism
MH  - Purine Nucleotides/*analysis
MH  - Rats
EDAT- 1983/10/14 00:00
MHDA- 1983/10/14 00:01
CRDT- 1983/10/14 00:00
PHST- 1983/10/14 00:00 [pubmed]
PHST- 1983/10/14 00:01 [medline]
PHST- 1983/10/14 00:00 [entrez]
AID - 10.1016/s0378-4347(00)84830-6 [doi]
PST - ppublish
SO  - J Chromatogr. 1983 Oct 14;277:137-44. doi: 10.1016/s0378-4347(00)84830-6.