PMID- 6643795
OWN - NLM
STAT- MEDLINE
DCOM- 19840107
LR  - 20151119
IS  - 0746-3898 (Print)
IS  - 0746-3898 (Linking)
VI  - 9
IP  - 1
DP  - 1983
TI  - Properties of cytosolic proteins that confer vasoactive intestinal 
      polypeptide-sensitivity to rat brain adenylate cyclase.
PG  - 19-34
AB  - Rat brain cortex, caudate nucleus and cerebellum contain one or more factors that 
      confer Vasoactive Intestinal Polypeptide (VIP)-, dopamine (DA)- and 
      norepinephrine (NE)-sensitivity to adenylate cyclase in the absence of added GTP. 
      These factors also stimulate the basal activity of the enzyme. The activity is 
      found in a 100 000 X g supernatant; has an apparent molecular weight greater than 
      450 000 daltons; is inactivated by pronase, alkaline phosphatase and ammonium 
      sulphate, and partially degraded by trypsin; and is stable to heat and acidic 
      treatment. The effect of the factors is additive with that of 
      guanosine-5'-triphosphate (GTP), and is not abolished by guanosine 
      5'-0-(2-thiodiphosphate) (GDP-beta-S). These results suggest that stimulation of 
      adenylate cyclase by VIP, and most likely by DA and NE, can be modulated by 
      soluble proteins.
FAU - Nicosia, S
AU  - Nicosia S
FAU - Borghi, C
AU  - Borghi C
FAU - Giachetti, A
AU  - Giachetti A
FAU - Oliva, D
AU  - Oliva D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cyclic Nucleotide Protein Phosphor Res
JT  - Journal of cyclic nucleotide and protein phosphorylation research
JID - 8309334
RN  - 0 (Nerve Tissue Proteins)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - VTD58H1Z2X (Dopamine)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Brain/*metabolism
MH  - Cytosol/*metabolism
MH  - Dialysis
MH  - Dopamine/pharmacology
MH  - Enzyme Activation/drug effects
MH  - Guanosine Triphosphate/pharmacology
MH  - Male
MH  - Molecular Weight
MH  - Nerve Tissue Proteins/*physiology
MH  - Norepinephrine/pharmacology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Vasoactive Intestinal Peptide/*pharmacology
EDAT- 1983/01/01 00:00
MHDA- 1983/01/01 00:01
CRDT- 1983/01/01 00:00
PHST- 1983/01/01 00:00 [pubmed]
PHST- 1983/01/01 00:01 [medline]
PHST- 1983/01/01 00:00 [entrez]
PST - ppublish
SO  - J Cyclic Nucleotide Protein Phosphor Res. 1983;9(1):19-34.