PMID- 6649404
OWN - NLM
STAT- MEDLINE
DCOM- 19840107
LR  - 20190829
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 8
IP  - 5
DP  - 1983 Oct
TI  - Hemagglutinating and hydrophobic surface properties of salmonellae producing 
      enterotoxin neutralized by cholera anti-toxin.
PG  - 443-58
AB  - Eleven Salmonella strains known to produce enterotoxin under aerobic culture 
      conditions in deferrated (DF) medium at 37 degrees C were shown to produce 
      enterotoxin with and without aeration at 22, 28, 37 and 42 degrees C. Heat-labile 
      enterotoxin was generally produced with growth temperatures up to 37 degrees C 
      irrespective of aeration. Heat-stable enterotoxin was produced up to 42 degrees 
      C, mainly aerobically, as indicated by infant mouse assay (IMA), by six of the 
      eleven strains tested. Nine strains produced heat-stable rapid permeability 
      factor (RPF) in rabbit skin. Cholera anti-toxin neutralized reactivities of 
      Salmonella heat-labile enterotoxin in four different biological assays. Mixed 
      gangliosides also neutralized this activity in the cell-test systems. With 
      guinea-pig erythrocytes, all strains underwent mannose-resistant hemagglutination 
      (MRHA) irrespective of growth temperatures, i.e. 22 and 37 degrees C or medium, 
      i.e., DF, tryptose soy broth (TSB) and colonization factor antigen (CFA) agar. At 
      both growth temperatures, CFA agar-grown cells of each strain caused MRHA of 
      bovine erythrocytes. Excepting three Salmonella typhimurium strains, DF 
      broth-grown cells gave MRHA of bovine, chicken and human group A erythrocytes, 
      CFA agar-grown cells caused MRHA of chicken and human blood, whereas TSB-grown 
      cells caused few MRHA reactions. Salmonellae producing both heat-stable, (ST) and 
      heat-labile, (LT) enterotoxins adsorbed to Phenyl Sepharose whereas salmonellae 
      that produced only LT enterotoxin did not. The presence of MRHA adhesions did not 
      correlate with cell-surface hydrophobicity. However, mannose-resistant 
      hemagglutinins may occur more commonly among salmonellae than has been previously 
      recognized.
FAU - Jiwa, S F
AU  - Jiwa SF
FAU - Mansson, I
AU  - Mansson I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antitoxins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Endotoxins)
RN  - 0 (Enterotoxins)
RN  - 0 (salmonella toxin)
RN  - 9012-63-9 (Cholera Toxin)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - Animals
MH  - Antitoxins/*immunology
MH  - Bacterial Toxins/analysis/*biosynthesis
MH  - Cholera Toxin/*immunology
MH  - Chromatography
MH  - *Endotoxins
MH  - Enterotoxins/analysis/*biosynthesis
MH  - Guinea Pigs
MH  - *Hemagglutination
MH  - Humans
MH  - Mannose/pharmacology
MH  - Rabbits
MH  - Salmonella/metabolism/*physiology
MH  - Surface Properties
EDAT- 1983/10/01 00:00
MHDA- 1983/10/01 00:01
CRDT- 1983/10/01 00:00
PHST- 1983/10/01 00:00 [pubmed]
PHST- 1983/10/01 00:01 [medline]
PHST- 1983/10/01 00:00 [entrez]
AID - 0378-1135(83)90039-1 [pii]
AID - 10.1016/0378-1135(83)90039-1 [doi]
PST - ppublish
SO  - Vet Microbiol. 1983 Oct;8(5):443-58. doi: 10.1016/0378-1135(83)90039-1.