PMID- 6657330
OWN - NLM
STAT- MEDLINE
DCOM- 19840214
LR  - 20190913
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 17
IP  - 12
DP  - 1983 Dec
TI  - Newborn polymorphonuclear leukocyte aggregation: a study of physical properties 
      and ultrastructure using chemotactic peptides.
PG  - 993-7
AB  - N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) and 
      N-formyl-L-methionyl-L-phenylalanine (FMP) were used to investigate neutrophil 
      (PMN) aggregation. Neutrophils were isolated from healthy adult volunteers and 
      term newborn cord blood. Neutrophil aggregation was measured after the addition 
      of FMLP and FMP. Adult PMN aggregation curves demonstrated initial aggregation 
      with slow deaggregation. Newborn neutrophil aggregation curves showed slow 
      aggregation with no deaggregation. These results were identical to the adult and 
      newborn neutrophil aggregation curves produced by C5a. Newborn PMN aggregates 
      examined by scanning electromicrography showed frequent, dense aggregates 
      compared with fewer, less dense aggregates of adult PMNs. Adult and newborn PMN 
      aggregates differed when compared by transmission electromicrographs (EM). 
      Newborn PMNs were tightly bound with cell membrane projections; adult PMNs were 
      loosely bound with no cell membrane projections. Cytochalasin-B pretreated adult 
      and newborn PMN aggregates displayed close approximation of cell membranes with 
      large numbers of cytoplasmic projections. Newborn neutrophils are irreversibly 
      aggregated by FMLP and FMP whereas adult neutrophils display an 
      aggregation-deaggregation reaction. EM studies suggest that this irreversible 
      aggregation of untreated newborn neutrophils may differ from the irreversible 
      aggregation of cytochalasin-B pretreated neutrophils.
FAU - Olson, T A
AU  - Olson TA
FAU - Ruymann, F B
AU  - Ruymann FB
FAU - Cook, B A
AU  - Cook BA
FAU - Burgess, D P
AU  - Burgess DP
FAU - Henson, S A
AU  - Henson SA
FAU - Thomas, P J
AU  - Thomas PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Chemotactic Factors)
RN  - 0 (Complement C5)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 59881-05-9 (N-formylmethionyl-methionyl-phenylalanine)
RN  - 80295-54-1 (Complement C5a)
SB  - IM
MH  - Adult
MH  - *Chemotactic Factors
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Complement C5
MH  - Complement C5a
MH  - Cytochalasin B/pharmacology
MH  - Humans
MH  - *Infant, Newborn
MH  - Microscopy, Electron, Scanning
MH  - N-Formylmethionine Leucyl-Phenylalanine/analogs & derivatives
MH  - Neutrophils/*physiology/ultrastructure
EDAT- 1983/12/01 00:00
MHDA- 1983/12/01 00:01
CRDT- 1983/12/01 00:00
PHST- 1983/12/01 00:00 [pubmed]
PHST- 1983/12/01 00:01 [medline]
PHST- 1983/12/01 00:00 [entrez]
AID - 10.1203/00006450-198312000-00013 [doi]
PST - ppublish
SO  - Pediatr Res. 1983 Dec;17(12):993-7. doi: 10.1203/00006450-198312000-00013.