PMID- 6658891
OWN - NLM
STAT- MEDLINE
DCOM- 19840224
LR  - 20190821
IS  - 0039-128X (Print)
IS  - 0039-128X (Linking)
VI  - 41
IP  - 4
DP  - 1983 Apr
TI  - Steroids in porcine follicular fluid: analysis by HPLC, capillary CG and 
      capillary CG/MS after purification on SEP-PAK C18 and ion exchange 
      chromatography.
PG  - 549-66
AB  - Steroids in porcine follicular fluid have been concentrated by reverse phase 
      chromatography in SEP-PAK C18 and purified further on the cation exchanger 
      SP-Sephadex C-25. Fractionation into unconjugated neutral and phenolic steroids, 
      glucuronides and sulfates was carried out on triethylaminohydroxypropyl Sephadex 
      LH-20 (TEAP-LH-20). The unconjugated neutral fraction was analysed by high 
      pressure liquid chromatography (HPLC) on a C18 radial cartridge 5 mm I.D.; 10 mu, 
      or on a C18 5 mu RESOLVE column, and by capillary gas chromatography (GC) on a 12 
      M OV-1 cross linked fused silica column. Testosterone, progesterone and 
      androstenedione were the major steroids detected by HPLC monitored at 254 nm, 
      although 17- hydroxy-, 20 alpha-dihydro- and 20 beta-dihydroprogesterone were 
      also present. Pregnenolone, pregnanediol, dehydroepiandrosterone, 
      17-hydroxypregnenolone and androsterone were detected by capillary CG as their 
      0-methyloxime trimethylsilyether derivatives. Further confirmation of structure 
      was provided by complete mass spectral data or by selective ion monitoring (SIM).
FAU - Khalil, M W
AU  - Khalil MW
FAU - Lawson, V
AU  - Lawson V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Steroids
JT  - Steroids
JID - 0404536
RN  - 0 (Steroids)
SB  - IM
MH  - Animals
MH  - Chromatography, Gas/methods
MH  - Chromatography, High Pressure Liquid/methods
MH  - Chromatography, Ion Exchange/methods
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry/methods
MH  - Ovarian Follicle/*analysis
MH  - Steroids/*analysis
MH  - Swine
EDAT- 1983/04/01 00:00
MHDA- 1983/04/01 00:01
CRDT- 1983/04/01 00:00
PHST- 1983/04/01 00:00 [pubmed]
PHST- 1983/04/01 00:01 [medline]
PHST- 1983/04/01 00:00 [entrez]
AID - 0039-128X(83)90094-6 [pii]
AID - 10.1016/0039-128x(83)90094-6 [doi]
PST - ppublish
SO  - Steroids. 1983 Apr;41(4):549-66. doi: 10.1016/0039-128x(83)90094-6.