PMID- 6660875
OWN - NLM
STAT- MEDLINE
DCOM- 19840224
LR  - 20220318
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 46
IP  - 6
DP  - 1983 Dec
TI  - Direct skin test in highly sensitized guinea pigs for rapid and sensitive 
      determination of staphylococcal enterotoxin B.
PG  - 1351-6
AB  - The direct skin test in highly sensitized guinea pigs was developed as a rapid 
      and extremely sensitive assay for detection of staphylococcal enterotoxin B (SEB) 
      in foods. This report details the experimental conditions required to elicit 
      optimal sensitization of guinea pigs to SEB. An intense and persistent 
      immunoglobulin E (IgE) anti-SEB response was established in strain 13 guinea pigs 
      pretreated with cyclophosphamide followed by four sensitizing doses of 10 
      micrograms of SEB 1 month apart. The conditions, however, optimal for eliciting 
      IgE responses led to a sustained failure to produce antibody of the IgG1 
      subclass. With the use of highly sensitized guinea pigs, one can achieve a 
      sensitivity ranging from 0.1 to 1.0 pg of purified SEB by the direct skin test 
      for at least 7 months after the last challenge. For analysis of SEB in food 
      extracts, the entire assay can be accomplished within 20 min with a sensitivity 
      of 10 to 100 pg SEB per ml of prepared food samples, and the recovery of 
      enterotoxin from spiked food products ranged between 75 and 89% of the amount 
      added.
FAU - Scheuber, P H
AU  - Scheuber PH
FAU - Mossmann, H
AU  - Mossmann H
FAU - Beck, G
AU  - Beck G
FAU - Hammer, D K
AU  - Hammer DK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Enterotoxins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 39424-53-8 (enterotoxin B, staphylococcal)
RN  - 8N3DW7272P (Cyclophosphamide)
SB  - IM
MH  - Biological Assay
MH  - Cyclophosphamide/pharmacology
MH  - Enterotoxins/*analysis/immunology
MH  - Food Contamination
MH  - Immunization
MH  - Immunoglobulin E/biosynthesis
MH  - Skin Tests
PMC - PMC239575
EDAT- 1983/12/01 00:00
MHDA- 1983/12/01 00:01
PMCR- 1983/12/01
CRDT- 1983/12/01 00:00
PHST- 1983/12/01 00:00 [pubmed]
PHST- 1983/12/01 00:01 [medline]
PHST- 1983/12/01 00:00 [entrez]
PHST- 1983/12/01 00:00 [pmc-release]
AID - 10.1128/aem.46.6.1351-1356.1983 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1983 Dec;46(6):1351-6. doi: 
      10.1128/aem.46.6.1351-1356.1983.