PMID- 6667315
OWN - NLM
STAT- MEDLINE
DCOM- 19840406
LR  - 20131121
IS  - 0037-8771 (Print)
IS  - 0037-8771 (Linking)
VI  - 59
IP  - 11
DP  - 1983 Nov 30
TI  - [Inhibition of heme biosynthesis in erythroid precursors (BFU-e) isolated from 
      human peripheral blood: effects on globin synthesis].
PG  - 1732-8
AB  - We studied the relationship between heme accumulation and globin synthesis in 
      human erythroid precursors which were stimulated by 2 I.U. of erythropoietin in 
      semi-solid cultures (1% methyl-cellulose, 20% fetal calf serum) and treated with 
      6-9 micrograms/ml of desferrioxamina (DF), a potent inhibitor of heme synthesis 
      (6). Heme accumulation was detected by specific reaction with benzidine (4), 
      globin synthesis by CM-cellulose column chromatography. Our results demonstrate 
      that globin gene expression occurs in DF-treated erythroid cells which do not 
      accumulate heme molecules. As heme does affect translation and stability of 
      globin mRNA (10) our system might be suitable for studies focused on pathological 
      alterations of erythropoiesis associated with the presence of unstable globin 
      mRNAs and/or unstable globins.
FAU - Barbieri, R
AU  - Barbieri R
FAU - Bernardi, F
AU  - Bernardi F
FAU - Buzzoni, D
AU  - Buzzoni D
FAU - Del Senno, L
AU  - Del Senno L
FAU - Gambari, R
AU  - Gambari R
FAU - Marchetti, G
AU  - Marchetti G
FAU - Perrotta, C M
AU  - Perrotta CM
FAU - Piva, M R
AU  - Piva MR
FAU - Conconi, F
AU  - Conconi F
LA  - ita
PT  - English Abstract
PT  - Journal Article
TT  - Inibizione della biosintesi dell'eme in precursori eritroidi (BFU-e) isolati da 
      sangue periferico umano: effetti sulla sin tesi globinica.
PL  - Italy
TA  - Boll Soc Ital Biol Sper
JT  - Bollettino della Societa italiana di biologia sperimentale
JID - 7506962
RN  - 11096-26-7 (Erythropoietin)
RN  - 42VZT0U6YR (Heme)
RN  - 9004-22-2 (Globins)
RN  - J06Y7MXW4D (Deferoxamine)
SB  - IM
MH  - Chromatography, Ion Exchange
MH  - Deferoxamine/pharmacology
MH  - Erythroblasts/drug effects/*metabolism
MH  - Erythropoietin/pharmacology
MH  - Globins/*biosynthesis
MH  - Heme/*biosynthesis
MH  - Humans
MH  - Kinetics
MH  - Protein Biosynthesis/drug effects
EDAT- 1983/11/30 00:00
MHDA- 1983/11/30 00:01
CRDT- 1983/11/30 00:00
PHST- 1983/11/30 00:00 [pubmed]
PHST- 1983/11/30 00:01 [medline]
PHST- 1983/11/30 00:00 [entrez]
PST - ppublish
SO  - Boll Soc Ital Biol Sper. 1983 Nov 30;59(11):1732-8.