PMID- 6673794
OWN - NLM
STAT- MEDLINE
DCOM- 19840607
LR  - 20191023
IS  - 0248-4900 (Print)
IS  - 0248-4900 (Linking)
VI  - 48
IP  - 2-3
DP  - 1983
TI  - Recovery of acetylcholinesterase and of its multiple molecular forms in motor 
      end-plate-free and motor end-plate-rich regions of mouse striated muscle, after 
      irreversible inactivation by an organophosphorus compound 
      (methyl-phosphorothiolate derivative).
PG  - 151-7
AB  - Most of mouse diaphragm muscle acetylcholinesterase (AChE) is irreversibly 
      inhibited after a single intraperitoneal injection of a methyl-phosphorothiolate 
      derivative (MPT), an organophosphorus compound which phosphorylates the active 
      site. The muscle recovers its AChE (de novo synthesis) and we studied the time 
      course of reappearance of AChE and its multiple active molecular forms. After 
      inhibition, there is an initial (3 to 15 hr) rapid recovery of total AChE (which 
      evolves from 20-28% to 50-60% of the control values), followed by a slow phase of 
      AChE return. After 3 days, the recovery is still incomplete (reaching 70-80% of 
      control values). Among the main molecular forms present in diaphragm muscle (16 
      S, 10 S and 4 S, accompanied by minor components), the 16 S and 10 S forms are 
      the most sensitive to MPT treatment. During the rapid initial phase of AChE 
      recovery, the absolute rate of recovery of the 4 S form is faster than for the 
      other forms with a correspondingly much higher relative proportion to total AChE. 
      These observations are consistent with the hypothesized precursor role of the 4 S 
      form. The 16 S form, which is found concentrated in the motor end-plate 
      (MEP)-rich regions and in low amounts in MEP-free regions, is similarly partially 
      recovered in both regions, suggesting that there is 16 S biosynthesis not only in 
      the MEP-rich regions but also in the MEP-free regions.
FAU - Goudou, D
AU  - Goudou D
FAU - Rieger, F
AU  - Rieger F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biol Cell
JT  - Biology of the cell
JID - 8108529
RN  - 0 (Cholinesterase Inhibitors)
RN  - 0 (Cholinesterase Reactivators)
RN  - 0 (Organothiophosphorus Compounds)
RN  - 9A4381183B (VX)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
SB  - IM
MH  - Acetylcholinesterase/*metabolism
MH  - Animals
MH  - Cholinesterase Inhibitors/*pharmacology
MH  - Cholinesterase Reactivators
MH  - Diaphragm/metabolism
MH  - Kinetics
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Motor Endplate/*enzymology
MH  - Muscles/enzymology
MH  - Neuromuscular Junction/*enzymology
MH  - Organothiophosphorus Compounds/*pharmacology
EDAT- 1983/01/01 00:00
MHDA- 1983/01/01 00:01
CRDT- 1983/01/01 00:00
PHST- 1983/01/01 00:00 [pubmed]
PHST- 1983/01/01 00:01 [medline]
PHST- 1983/01/01 00:00 [entrez]
AID - 10.1111/j.1768-322x.1984.tb00209.x [doi]
PST - ppublish
SO  - Biol Cell. 1983;48(2-3):151-7. doi: 10.1111/j.1768-322x.1984.tb00209.x.