PMID- 6674450
OWN - NLM
STAT- MEDLINE
DCOM- 19840618
LR  - 20071114
IS  - 0022-3395 (Print)
IS  - 0022-3395 (Linking)
VI  - 69
IP  - 6
DP  - 1983 Dec
TI  - Lysosomal enzyme activities in susceptible and refractory strains of Biomphalaria 
      glabrata during the course of infection with Schistosoma mansoni.
PG  - 1018-26
AB  - The distribution and abundance of the lysosomal enzyme markers, acid phosphatase 
      (AP), peroxidase (PO), and nonspecific esterase (NE), within circulating blood 
      cells (hemocytes) were examined in a schistosome-susceptible (PR albino M-line) 
      and a resistant (10-R2) strain of Biomphalaria glabrata during the course of 
      infection with Schistosoma mansoni. The dynamics of serum (cell-free hemolymph) 
      AP activities and total hemocyte numbers in infected snails also were 
      investigated. Hemocyte subpopulations, as determined by these enzyme markers, 
      responded differently to parasite infection between snail strains. Generally, the 
      hemocyte subpopulations within PR albino snails remained largely unchanged, 
      whereas the same subpopulations in 10-R2 snails fluctuated considerably. The 
      distribution of AP in the hemocytes of 10-R2 snails decreased by 1 hr 
      postexposure (PE) to the parasite and remained low through 12 hr before 
      increasing to control values at 24 hr and 2 wk PE. In comparison, PO activity 
      increased by 1 hr PE and peaked at 12 hr before dropping to 0 hr values by 2 wk 
      PE. The NE activity exhibited still another pattern with the percentage of 
      NE-positive cells decreasing from 0 to 12 hr PE followed by a recovery to 0-hr 
      values by 24 hr. The abundance of these hemocyte enzymes followed a similar 
      pattern to that of their distribution, although some differences were observed. 
      Serum AP values varied little in PR albino snails except for a significant 
      increase at 2 wk PE, indicating a possible response to tissue damage resulting 
      from migrating daughter sporocysts.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Granath, W O Jr
AU  - Granath WO Jr
FAU - Yoshino, T P
AU  - Yoshino TP
LA  - eng
GR  - AI02656/AI/NIAID NIH HHS/United States
GR  - AI15503-05/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Parasitol
JT  - The Journal of parasitology
JID - 7803124
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 3.1.1.- (Carboxylic Ester Hydrolases)
RN  - EC 3.1.1.1 (Carboxylesterase)
RN  - EC 3.1.3.2 (Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/*metabolism
MH  - Animals
MH  - Biomphalaria/enzymology/*parasitology
MH  - Carboxylesterase
MH  - Carboxylic Ester Hydrolases/*metabolism
MH  - Disease Susceptibility
MH  - Kinetics
MH  - Lysosomes/*enzymology
MH  - Peroxidases/*metabolism
MH  - Schistosoma mansoni/*pathogenicity
MH  - Species Specificity
EDAT- 1983/12/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1983/12/01 00:00
PHST- 1983/12/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1983/12/01 00:00 [entrez]
PST - ppublish
SO  - J Parasitol. 1983 Dec;69(6):1018-26.