PMID- 6697476
OWN - NLM
STAT- MEDLINE
DCOM- 19840330
LR  - 20191023
IS  - 0009-9090 (Print)
IS  - 0009-9090 (Linking)
VI  - 14
IP  - 1
DP  - 1984 Jan
TI  - The diversity of allergens involved in bakers' asthma.
PG  - 93-107
AB  - Sera from 35 individuals with suspected allergies to inhaled flour were screened 
      for the presence of immunoglobulin E (IgE) specific for wheat-flour proteins. 
      Sera from nine asthmatic bakers with high wheat RAST scores were selected for 
      further study with the aim of purifying the allergen(s) involved in bakers' 
      asthma and related conditions. However, each of the different techniques 
      applied--ion exchange chromatography, preparative isoelectric focusing and the 
      electrophoretic transfer, or 'Western blotting' technique, showed that serum IgE 
      from different individuals have markedly different specificities and bind to 
      numerous wheat proteins. When three purified wheat proteins were tested--wheat 
      germ agglutinin; a fraction purified using a concanavalin-A affinity column and a 
      putative trypsin inhibitor--all were identified as allergens for some but not all 
      of the allergic bakers.
FAU - Sutton, R
AU  - Sutton R
FAU - Skerritt, J H
AU  - Skerritt JH
FAU - Baldo, B A
AU  - Baldo BA
FAU - Wrigley, C W
AU  - Wrigley CW
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Allergy
JT  - Clinical allergy
JID - 0311172
RN  - 0 (Allergens)
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Allergens/*immunology
MH  - Asthma/*immunology
MH  - Chromatography, Ion Exchange
MH  - Cooking
MH  - Electrophoresis
MH  - Female
MH  - Flour/*adverse effects
MH  - Humans
MH  - Isoelectric Focusing
MH  - Male
MH  - Occupational Diseases/*immunology
MH  - Plant Proteins/isolation & purification
MH  - Triticum
EDAT- 1984/01/01 00:00
MHDA- 1984/01/01 00:01
CRDT- 1984/01/01 00:00
PHST- 1984/01/01 00:00 [pubmed]
PHST- 1984/01/01 00:01 [medline]
PHST- 1984/01/01 00:00 [entrez]
AID - 10.1111/j.1365-2222.1984.tb02196.x [doi]
PST - ppublish
SO  - Clin Allergy. 1984 Jan;14(1):93-107. doi: 10.1111/j.1365-2222.1984.tb02196.x.