PMID- 6698126
OWN - NLM
STAT- MEDLINE
DCOM- 19840417
LR  - 20060417
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 12
IP  - 1
DP  - 1984 Jan
TI  - Hematopoiesis on cellulose-ester membranes (CEM). VI. Histochemical evaluation of 
      stromal-CEM interactions after stromal enrichment.
PG  - 25-30
AB  - Histochemical procedures were used to elucidate differences in the distribution 
      of glycosaminoglycans (GAGs) and glycoproteins (GPs) in tubes made of 
      cellulose-ester membrane (CEM) in six weeks and 12 months following 
      intraperitoneal (i.p.) implantation. After six weeks, CEM tubes coated on their 
      inner surfaces with bone marrow (BM), bone homogenate (BH), or regenerating 
      medullary mesenchyme (M) contained a highly vascularized loose connective tissue 
      and scattered hematopoietic elements. Numerous mononuclear cells were seen at the 
      stromal-CEM interface and infiltrating the CEM. At the site of infiltration, 
      there were accumulations of GPs and nonsulfated acidic GAGs. The remainder of the 
      CEM contained limited amounts of sulfated and nonsulfated GAGs. By 12 months, 
      both coated and uncoated CEMs were saturated with sulfated and nonsulfated acidic 
      GAGs. However, only coated CEM tubes contained trilineal hematopoiesis surrounded 
      by a shell of bone that was incorporated into the structure of the CEM. Exterior 
      to the bone, the CEM was infiltrated with GPs, some of which were acidic and 
      nonsulfated, possibly sialoglycoproteins. No bone or glycoproteins infiltrated 
      the uncoated CEMs. The stroma supporting trilineal hematopoiesis contained GPs 
      and nonsulfated acidic GAGs, but not sulfated acidic GAGs. However, both sulfated 
      and nonsulfated GAGs were found in the fibrous connective tissue filling the 
      lumens of uncoated CEMs. While CEM tubes accumulate peritoneal GAGs that may be 
      conducive for the retention, differentiation, and proliferation of osteogenic and 
      other stromal cells provided by coatings of BM, BH, or M, it is these cells that 
      produce the bone and glycoproteins that appear to contribute to a 
      microenvironment conducive to trilineal hematopoiesis. However, the relative 
      roles of these substances in this process remain to be elucidated.
FAU - McCuskey, R S
AU  - McCuskey RS
FAU - Meineke, H A
AU  - Meineke HA
FAU - Pinkstaff, C A
AU  - Pinkstaff CA
FAU - Knospe, W H
AU  - Knospe WH
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Glycoproteins)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Membranes, Artificial)
RN  - 0 (cellulose ester plastic)
RN  - 9004-34-6 (Cellulose)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cellulose/analogs & derivatives
MH  - Connective Tissue Cells
MH  - Glycoproteins/analysis
MH  - Glycosaminoglycans/analysis
MH  - *Hematopoiesis
MH  - *Membranes, Artificial
MH  - Mice
EDAT- 1984/01/01 00:00
MHDA- 1984/01/01 00:01
CRDT- 1984/01/01 00:00
PHST- 1984/01/01 00:00 [pubmed]
PHST- 1984/01/01 00:01 [medline]
PHST- 1984/01/01 00:00 [entrez]
PST - ppublish
SO  - Exp Hematol. 1984 Jan;12(1):25-30.