PMID- 6711762
OWN - NLM
STAT- MEDLINE
DCOM- 19840524
LR  - 20141120
IS  - 0192-8562 (Print)
IS  - 0192-8562 (Linking)
VI  - 6
IP  - 1
DP  - 1984 Spring
TI  - Non-uniformity of intracellular polymer formation in sickle erythrocytes: 
      possible correlation with severity of hemolytic anemia.
PG  - 46-50
AB  - Intact sickle erythrocytes were fractionated to obtain a more uniform population 
      in mean corpuscular hemoglobin concentration (MCHC). Polymer fraction at complete 
      deoxygenation varied with increasing MCHC and, at varying oxygen saturation, was 
      in good agreement with theoretical predictions based on solubility and the 
      non-ideal behavior of concentrated hemoglobin solution and of water. These data 
      and the theoretical analysis indicate that, in uniform sickle erythrocytes at 34 
      g/dl, polymer is only detected below 84% oxygen saturation. Non-uniformity of 
      unfractionated sickle erythrocytes causes this critical oxygen saturation to 
      shift to higher values (over 90%). The implication is that polymer may exist in 
      arterial blood, a possibility which must be considered in therapeutic strategies. 
      Recently, we showed that polymer formation correlated with the severity of 
      hemolysis for 12 sickle syndromes varying from sickle trait to hemoglobin SS 
      (African) disease.
FAU - Noguchi, C T
AU  - Noguchi CT
FAU - Schechter, A N
AU  - Schechter AN
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pediatr Hematol Oncol
JT  - The American journal of pediatric hematology/oncology
JID - 7908071
RN  - 0 (Hemoglobin, Sickle)
SB  - IM
MH  - Anemia, Sickle Cell/*blood/pathology
MH  - Cell Fractionation
MH  - Erythrocyte Indices
MH  - Erythrocytes, Abnormal/*metabolism
MH  - Hemoglobin, Sickle/*metabolism
MH  - Humans
MH  - In Vitro Techniques
EDAT- 1984/01/01 00:00
MHDA- 1984/01/01 00:01
CRDT- 1984/01/01 00:00
PHST- 1984/01/01 00:00 [pubmed]
PHST- 1984/01/01 00:01 [medline]
PHST- 1984/01/01 00:00 [entrez]
PST - ppublish
SO  - Am J Pediatr Hematol Oncol. 1984 Spring;6(1):46-50.