PMID- 6714849
OWN - NLM
STAT- MEDLINE
DCOM- 19840607
LR  - 20211203
IS  - 0015-5691 (Print)
IS  - 0015-5691 (Linking)
VI  - 83
IP  - 1
DP  - 1984 Jan
TI  - [The factors affecting plasma catecholamines concentration in rats and man].
PG  - 17-31
AB  - Rat and human plasma catecholamines were measured simultaneously by HPLC-THI, 
      HPLC-ECD and REA, and the three methods were compared. An attempt was also made 
      to determine the factors affecting the estimated value of plasma catecholamine 
      concentration. Our study showed that: Sensitivity and reproducibility to 
      norepinephrine and epinephrine were identical in all three methods. One advantage 
      of the REA method is that comparatively smaller sample volumes are required to 
      produce similar results. Plasma dopamine concentration in peripheral blood 
      samples was determined by the HPLC-ECD rather than the HPLC-THI method. 
      Withdrawal of 5 ml of blood produced a significant increase in norepinephrine, 
      epinephrine and dopamine in rat plasma. The catecholamine concentration in these 
      cases was determined by the REA method. Plasma norepinephrine concentration did 
      not increase with age in Wistar Kyoto rats. However, plasma norepinephrine 
      concentration increased significantly with age in stroke-prone spontaneously 
      hypertensive rats (SHRSP). Plasma norepinephrine concentration in male SHRSP was 
      greater than that in female SHRSP. SHRSP-plasma norepinephrine concentrations 
      rose in parallel to increases in blood pressure. The plasma norepinephrine 
      concentration in SHRSP with cerebral hemorrhage rose significantly as compared 
      with the plasma norepinephrine levels in SHRSP without cerebral bleeding. Because 
      each method of determination of plasma catecholamine concentration has both 
      merits and demerits, selection should be determined by sample size and amount of 
      catecholamines in the plasma samples. Factors affecting the estimated value of 
      plasma catecholamine concentration should be taken into consideration.
FAU - Minami, M
AU  - Minami M
FAU - Sano, M
AU  - Sano M
FAU - Togashi, H
AU  - Togashi H
FAU - Endo, T
AU  - Endo T
FAU - Saito, I
AU  - Saito I
FAU - Nomura, A
AU  - Nomura A
FAU - Saito, H
AU  - Saito H
FAU - Nakamura, N
AU  - Nakamura N
FAU - Kurimoto, F
AU  - Kurimoto F
FAU - Sakurai, H
AU  - Sakurai H
AU  - et al.
LA  - jpn
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - Nihon Yakurigaku Zasshi
JT  - Nihon yakurigaku zasshi. Folia pharmacologica Japonica
JID - 0420550
RN  - 0 (Catecholamines)
RN  - 0 (Indoles)
RN  - 0 (PHB2 protein, human)
RN  - 0 (Prohibitins)
RN  - 28986-53-0 (trihydroxyindole)
RN  - VTD58H1Z2X (Dopamine)
RN  - X4W3ENH1CV (Norepinephrine)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Animals
MH  - Catecholamines/*blood
MH  - Chromatography, High Pressure Liquid/methods
MH  - Dopamine/blood
MH  - Electrochemistry/methods
MH  - Epinephrine/blood
MH  - Hypertension/*blood
MH  - Indoles
MH  - Norepinephrine/blood
MH  - Prohibitins
MH  - Radioligand Assay
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1984/01/01 00:00
MHDA- 1984/01/01 00:01
CRDT- 1984/01/01 00:00
PHST- 1984/01/01 00:00 [pubmed]
PHST- 1984/01/01 00:01 [medline]
PHST- 1984/01/01 00:00 [entrez]
PST - ppublish
SO  - Nihon Yakurigaku Zasshi. 1984 Jan;83(1):17-31.