PMID- 6724015
OWN - NLM
STAT- MEDLINE
DCOM- 19840720
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 42
IP  - 1
DP  - 1984 Jul
TI  - A test for the practical evaluation of male fertility by acridine orange (AO) 
      fluorescence.
PG  - 87-91
AB  - A new and practical test for evaluating the fertility of a male subject has been 
      developed. Twenty-eight donors whose semen had induced at least one pregnancy 
      resulting in a normal delivery and 61 patients attending our infertility clinic 
      were studied. Semen smears stained with acridine orange were read on a 
      fluorescence microscope; sperm heads appeared either green (fertile) or red ( 
      nonfertile ). We introduce the concept of an "effective sperm count" which is 
      obtained by multiplying the percentage of green-fluorescing sperm by the actual 
      sperm count. Of our fertile subjects, 27 of 28 (96.4%) exhibited an effective 
      sperm count of greater than or equal to 50 million/ml, while 60 of 61 (98.3%) 
      infertile patients fell below this value. The percent green correlates with 
      neither actual sperm count nor motility, indicating that this test measures a new 
      parameter of male fertility.
FAU - Tejada, R I
AU  - Tejada RI
FAU - Mitchell, J C
AU  - Mitchell JC
FAU - Norman, A
AU  - Norman A
FAU - Marik, J J
AU  - Marik JJ
FAU - Friedman, S
AU  - Friedman S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Humans
MH  - Infertility, Male/*diagnosis/pathology/physiopathology
MH  - Male
MH  - Microscopy, Fluorescence
MH  - *Sperm Count
MH  - Sperm Head/*pathology
MH  - Spermatozoa/*pathology
EDAT- 1984/07/01 00:00
MHDA- 1984/07/01 00:01
CRDT- 1984/07/01 00:00
PHST- 1984/07/01 00:00 [pubmed]
PHST- 1984/07/01 00:01 [medline]
PHST- 1984/07/01 00:00 [entrez]
AID - S0015-0282(16)47963-X [pii]
AID - 10.1016/s0015-0282(16)47963-x [doi]
PST - ppublish
SO  - Fertil Steril. 1984 Jul;42(1):87-91. doi: 10.1016/s0015-0282(16)47963-x.