PMID- 6731751
OWN - NLM
STAT- MEDLINE
DCOM- 19840713
LR  - 20190821
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 39
IP  - 4
DP  - 1984 May
TI  - Bronchial reactivity in relation to immune responses in rats after subcutaneous 
      sensitization without adjuvant.
PG  - 249-58
AB  - Rats of BNxWi /Fu strain were immunized by daily subcutaneous (s.c.) injections 
      of antigen without the use of adjuvant for two 2-week periods with a 4-week 
      interval. The bronchial responses to airway and intravenous (i.v.) antigen 
      challenge were measured during and after the immunization period. These responses 
      were compared with both the humoral and the cell-mediated immune response of the 
      animals. Immunization induced bronchial reactivity of the animals to antigen 
      after airway and i.v. challenge. This reactivity persisted for 7 weeks following 
      the second immunization period. Specific IgE antibodies were detected in serum, 
      bronchial fluid and in supernatants from cultured peripheral lymph node cells. 
      The immunization also resulted in an IgG antibody response. Neither the amount of 
      IgE, IgG2a, nor of any other isotype, correlated with the bronchial reactivity in 
      the animals. Antigen stimulation of lymph node cells mixed with syngeneic spleen 
      cells induced proliferation. This procedure also resulted in a maturation of mast 
      cells in 3-week cultures. The immunization also resulted in some increase in the 
      number of mast cells around vessels in the lung. There was no correlation between 
      these parameters of cell-mediated immunity and either antibody responses or 
      bronchial reactivity.
FAU - Smedegard, G
AU  - Smedegard G
FAU - Ahlstedt, S
AU  - Ahlstedt S
FAU - Bjorksten, B
AU  - Bjorksten B
FAU - Nygren, H
AU  - Nygren H
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage
MH  - Animals
MH  - Antibody Formation
MH  - Blood Gas Analysis
MH  - Blood Pressure
MH  - Bronchi/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Immunity, Cellular
MH  - *Immunization
MH  - Immunoglobulin E/*analysis
MH  - Immunoglobulin G/analysis
MH  - Lung/immunology/pathology
MH  - Lymphocytes/immunology
MH  - Male
MH  - Mast Cells/cytology/immunology
MH  - Radioallergosorbent Test
MH  - Rats
MH  - Respiration
MH  - Respiratory Hypersensitivity/*immunology
EDAT- 1984/05/01 00:00
MHDA- 1984/05/01 00:01
CRDT- 1984/05/01 00:00
PHST- 1984/05/01 00:00 [pubmed]
PHST- 1984/05/01 00:01 [medline]
PHST- 1984/05/01 00:00 [entrez]
AID - 10.1111/j.1398-9995.1984.tb00862.x [doi]
PST - ppublish
SO  - Allergy. 1984 May;39(4):249-58. doi: 10.1111/j.1398-9995.1984.tb00862.x.