PMID- 6733273
OWN - NLM
STAT- MEDLINE
DCOM- 19840730
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 64
IP  - 1
DP  - 1984 Jul
TI  - The response of human neutrophils to a chemotactic tripeptide 
      (N-formyl-methionyl-leucyl-phenylalanine) studied by microcinematography.
PG  - 221-8
AB  - A quantitative microcinematographic technique for the measurement of cellular 
      motion in response to chemical influences is described. The data are analyzed by 
      using a magnetic digitizer connected to a computer, permitting comparison of 
      several characteristics of such motion. When human granulocytes are placed in a 
      gradient of formyl-methionyl-leucyl-phenylalanine (fMLP) (concentration 
      10(-6)-10(-8) mol/L) against buffer, the motion of the cells is increased in 
      velocity (chemokinesis) and is directed toward the chemoattractant, as measured 
      by the orientation of the vector of motion. This directionality is confirmed by 
      positive values for the McCutcheon index and analysis of directed linear 
      displacement. Concentrations of fMLP below 10(-9) mol/L did not result in 
      chemokinesis or chemotaxis, presumably due to insufficient stimulus. 
      Concentrations of 10(-6) mol/L fMLP and greater resulted in marked stimulation of 
      the cell surface but reduced directionality and velocity of motion compared with 
      cells in a more optimal gradient. Motion of the unstimulated cells tended to be 
      ortholinear , and this was not increased by the presence of a gradient of fMLP. 
      Hence, as previously shown, the Random Walk analysis, which does not give weight 
      to the direction of motion, is not useful in the analysis of the response of 
      these cells to a chemotaxin in this system.
FAU - Maher, J
AU  - Maher J
FAU - Martell, J V
AU  - Martell JV
FAU - Brantley, B A
AU  - Brantley BA
FAU - Cox, E B
AU  - Cox EB
FAU - Niedel, J E
AU  - Niedel JE
FAU - Rosse, W F
AU  - Rosse WF
LA  - eng
GR  - AI 18308/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Cell Movement/drug effects
MH  - Chemotaxis
MH  - Humans
MH  - Motion Pictures
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/*drug effects
MH  - Time Factors
EDAT- 1984/07/01 00:00
MHDA- 1984/07/01 00:01
CRDT- 1984/07/01 00:00
PHST- 1984/07/01 00:00 [pubmed]
PHST- 1984/07/01 00:01 [medline]
PHST- 1984/07/01 00:00 [entrez]
AID - S0006-4971(20)83861-0 [pii]
PST - ppublish
SO  - Blood. 1984 Jul;64(1):221-8.