PMID- 6746120
OWN - NLM
STAT- MEDLINE
DCOM- 19840904
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 34
IP  - 1
DP  - 1984 Jul 15
TI  - Isoenzyme pattern and subcellular localization of hexokinases in human breast 
      cancer and nonpathological breast tissue.
PG  - 63-6
AB  - Subcellular distribution of hexokinase (HK) isoenzymes in 22 human breast cancers 
      (21 primary cancers and 1 axillary metastatic growth) and 7 non-pathological 
      human mammary gland tissue samples was studied with starch gel electrophoresis on 
      isolated cell fractions obtained by differential centrifugation. Fractions used 
      were cytosol, mitochondria and microsomes. A comparison of two methods for 
      detecting HK activity was made, yielding different results regarding HK II and HK 
      III. A method based on the ability of NADPH to fluoresce in UV gave a constant 
      pattern of HK isoenzymes. In non-pathological breast tissue, only HK I was seen, 
      i.e. in the cytosol and the microsomal fractions. HK I was also seen in all 
      fractions of the cancers, but another more anodal band of HK I, as well as HK II 
      and HK III, consistently appeared in the cytosol fractions. The more commonly 
      used staining technique with tetrazolium dye revealed HK II in 45% and HK III in 
      50% of the samples. The pattern of HK isoenzymes in the cancers was the same 
      irrespective of estrogen and progesterone cytosolic receptor contents and the 
      histology of the tumors. The fluorescence method is, therefore, much more 
      sensitive than the tetrazolium technique for detecting HK activity after 
      electrophoresis and could explain difference in results obtained by various 
      laboratories.
FAU - Gudnason, V
AU  - Gudnason V
FAU - Ingvarsson, S
AU  - Ingvarsson S
FAU - Jonasdottir, A
AU  - Jonasdottir A
FAU - Andresdottir, V
AU  - Andresdottir V
FAU - Egilsson, V
AU  - Egilsson V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Isoenzymes)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Breast/*enzymology
MH  - Breast Neoplasms/*enzymology
MH  - Cell Compartmentation
MH  - Hexokinase/metabolism
MH  - Humans
MH  - Isoenzymes/metabolism
MH  - Mitochondria/enzymology
MH  - Receptors, Estrogen/metabolism
MH  - Receptors, Progesterone/metabolism
EDAT- 1984/07/15 00:00
MHDA- 1984/07/15 00:01
CRDT- 1984/07/15 00:00
PHST- 1984/07/15 00:00 [pubmed]
PHST- 1984/07/15 00:01 [medline]
PHST- 1984/07/15 00:00 [entrez]
AID - 10.1002/ijc.2910340111 [doi]
PST - ppublish
SO  - Int J Cancer. 1984 Jul 15;34(1):63-6. doi: 10.1002/ijc.2910340111.