PMID- 6747638
OWN - NLM
STAT- MEDLINE
DCOM- 19840913
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 43
IP  - 3
DP  - 1984 Sep
TI  - Release of the NILE and other glycoproteins from cultured PC12 rat 
      pheochromocytoma cells and sympathetic neurons.
PG  - 841-8
AB  - Studies were carried out on the glycoproteins (GPs) released by cultured rat 
      sympathetic neurons and by cultured PC12 rat pheochromocytoma cells with and 
      without nerve growth factor (NGF) treatment. Cultures were prelabeled with 
      [3H]fucose and then incubated for 4-8 h in fresh unlabeled medium. The material 
      released into the medium was analyzed by sodium dodecyl sulfate-polyacrylamide 
      gel electrophoresis (SDS-PAGE) and fluorography. The patterns of labeled material 
      released by all three types of cultures were similar. One of the major components 
      released was of apparent Mr less than or equal to 230,000. Another major 
      component of apparent Mr = 55,000 as well as minor components of apparent Mr less 
      than or equal to 180,000, 140,000, 118,000, and 105,000 were also detected. An 
      additional peptide of apparent Mr less than or equal to 210,000 was released only 
      by the sympathetic neurons. The soluble released Mr less than or equal to 230,000 
      component appeared to be derived from a previously characterized neuronal 
      integral membrane GP referred to as the NILE (NGF-inducible large external) GP. 
      Evidence for this included recognition of the released component by a 
      monospecific antiserum prepared against membrane-derived NILE GP. At least 
      several of the other released GPs appeared to be derived from membrane-bound 
      components with which they share immuno-crossreactivity. Since the soluble NILE 
      and other released GPs had somewhat faster mobilities on SDS-polyacrylamide gels 
      than their apparent membrane-bound correspondents, release could either be due 
      to, or accompanied by, minor changes in molecular structure.
FAU - Richter-Landsberg, C
AU  - Richter-Landsberg C
FAU - Lee, V M
AU  - Lee VM
FAU - Salton, S R
AU  - Salton SR
FAU - Shelanski, M L
AU  - Shelanski ML
FAU - Greene, L A
AU  - Greene LA
LA  - eng
GR  - NS 15076/NS/NINDS NIH HHS/United States
GR  - NS 16036/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Glycoproteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neural Cell Adhesion Molecule L1)
SB  - IM
MH  - Adrenal Gland Neoplasms/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Ganglia, Sympathetic/*metabolism
MH  - Glycoproteins/isolation & purification/*metabolism
MH  - Molecular Weight
MH  - Nerve Growth Factors/*metabolism
MH  - Neural Cell Adhesion Molecule L1
MH  - Neurons/*metabolism
MH  - Pheochromocytoma/*metabolism
MH  - Rats
EDAT- 1984/09/01 00:00
MHDA- 1984/09/01 00:01
CRDT- 1984/09/01 00:00
PHST- 1984/09/01 00:00 [pubmed]
PHST- 1984/09/01 00:01 [medline]
PHST- 1984/09/01 00:00 [entrez]
AID - 10.1111/j.1471-4159.1984.tb12807.x [doi]
PST - ppublish
SO  - J Neurochem. 1984 Sep;43(3):841-8. doi: 10.1111/j.1471-4159.1984.tb12807.x.