PMID- 6749995
OWN - NLM
STAT- MEDLINE
DCOM- 19821202
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 52
IP  - 1
DP  - 1982
TI  - A solid-phase fluoroimmunoassay for human IgE.
PG  - 81-90
AB  - A fluoroimmunoassay (FIA) for the measurement of immunoglobulin E (IgE) is 
      described. The method involves a sandwich technique in which antiserum to human 
      IgE is adsorbed on a cellulose acetate/nitrate disc which is attached to a 
      plastic StiQTM sampler. The prepared sampler is reacted with serum and antigen is 
      bound specifically. After buffer wash and treatment with goat serum to block 
      non-specific binding, the samplers are reacted with monospecific fluorescein 
      conjugated antisera to human IgE. Two buffer washes remove unbound material and 
      the fluorescence which is directly proportional to the IgE concentration is 
      measured in a FIAX fluorometer. Assay standards range from 2 to 400 IU/ml. The 
      method gives within run coefficients of variation (CV) from 3.3 to 8.4% and 
      between run CV from 6.2 to 16.1% being less precise at low analyte 
      concentrations. IgE concentrations of a group of 74 sera determined by FIA using 
      StiQsTM prepared with antisera from 2 sources correlated well with results found 
      by radioimmunoassay.
FAU - Blanchard, G C
AU  - Blanchard GC
FAU - Gardner, R E
AU  - Gardner RE
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - *Fluorescent Antibody Technique
MH  - Goats
MH  - Humans
MH  - Immunoassay/methods
MH  - Immunoglobulin E/*analysis
MH  - Radioimmunoassay
MH  - Radioimmunosorbent Test
MH  - Sheep
EDAT- 1982/01/01 00:00
MHDA- 1982/01/01 00:01
CRDT- 1982/01/01 00:00
PHST- 1982/01/01 00:00 [pubmed]
PHST- 1982/01/01 00:01 [medline]
PHST- 1982/01/01 00:00 [entrez]
AID - 0022-1759(82)90352-0 [pii]
AID - 10.1016/0022-1759(82)90352-0 [doi]
PST - ppublish
SO  - J Immunol Methods. 1982;52(1):81-90. doi: 10.1016/0022-1759(82)90352-0.