PMID- 6759227
OWN - NLM
STAT- MEDLINE
DCOM- 19830311
LR  - 20190515
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 31
IP  - 2
DP  - 1982 Feb
TI  - Selective binding of fluoresceinated-avidin to A-cells of sectioned pancreas.
PG  - 107-9
AB  - Fluoresceinated-avidin (FITC-avidin) was observed to bind specifically to a 
      subset of pancreatic islet cells in sections of both human and rat pancreas. 
      FITC-avidin binding was inhibited by excess unlabeled avidin, and by biotin, but 
      not by glucagon, somatostatin, or insulin. Labeling of islets with anti-insulin, 
      anti-glucagon, anti-somatostatin, and anti-human pancreatic polypeptide 
      antibodies showed the avidin binding subset to correspond to islet cells 
      identified by anti-glucagon antibody. Conversely, avidin reacted with no insulin, 
      somatostatin, or cells containing HPP. We conclude that avidin localizes 
      specifically to A-islet cells. Binding may be to a biotin-containing enzyme 
      within the A-cells, but the precise molecular site of binding is currently 
      unidentified.
FAU - Rowley, R F
AU  - Rowley RF
FAU - Eisenbarth, G S
AU  - Eisenbarth GS
LA  - eng
GR  - AM 25778-01/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - 0 (Fluoresceins)
RN  - 0 (fluorescein isothiocyante avidin)
RN  - 1405-69-2 (Avidin)
RN  - 9006-59-1 (Ovalbumin)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Avidin/*analogs & derivatives
MH  - *Fluorescein-5-isothiocyanate/*analogs & derivatives
MH  - *Fluoresceins
MH  - Histocytochemistry
MH  - Humans
MH  - Islets of Langerhans/*cytology
MH  - Microscopy, Fluorescence
MH  - Ovalbumin/*analogs & derivatives
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Rats, Inbred Strains
EDAT- 1982/02/01 00:00
MHDA- 1982/02/01 00:01
CRDT- 1982/02/01 00:00
PHST- 1982/02/01 00:00 [pubmed]
PHST- 1982/02/01 00:01 [medline]
PHST- 1982/02/01 00:00 [entrez]
AID - 10.2337/diab.31.2.107 [doi]
PST - ppublish
SO  - Diabetes. 1982 Feb;31(2):107-9. doi: 10.2337/diab.31.2.107.