PMID- 6765857
OWN - NLM
STAT- MEDLINE
DCOM- 19900214
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 108
IP  - 2
DP  - 1982 Aug
TI  - Characterization of three macrophage chemotactic factors from PPD-induced delayed 
      hypersensitivity reaction sites in guinea pigs, with special reference to a 
      chemotactic lymphokine.
PG  - 171-83
AB  - Three types of macrophage chemotactic factors (MCFs) were separated from purified 
      protein derivative (PPD)-induced delayed hypersensitivity (DHR) skin lesions in 
      guinea pigs, and MCF-c was purified isotachophoretically. The macrophage 
      chemotactic activity (MCA) of the skin extract was mostly associated with MCF-a 
      and c. MCF-c (mol wt 110,000) seemed more significant for mediating the 
      macrophage reaction than MCF-a (mol wt 150,000), which exhibited common 
      antigenicity with serum IgG. MCF-b (mol wt 14,000), manifesting common 
      antigenicity with the fifth component of complement (C5), was apparently less 
      significant. MCF-c was thermostable and had an isoelectric point (pI) of 5.2 +/- 
      0.1. The protein exhibited marked macrophage chemotactic activities (MCA) but no 
      neutrophil chemotactic, lymphocyte chemotactic, and MIF activity in excess of 
      that in vitro. Selective accumulation of macrophages was observed in response to 
      intradermal MCF-c injection. Our studies suggest that MCF-c at 24-hour skin sites 
      of DHR may exist as specific lymphokine preparations with high potential for 
      monocyte infiltration.
FAU - Honda, M
AU  - Honda M
AD  - Department of Pathology, University of Kumamoto School of Medicine, Japan.
FAU - Hayashi, H
AU  - Hayashi H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Chemotactic Factors)
RN  - 0 (Lymphokines)
SB  - IM
MH  - Animals
MH  - Chemical Phenomena
MH  - Chemistry
MH  - Chemistry, Physical
MH  - Chemotactic Factors/immunology/*metabolism/physiology
MH  - Guinea Pigs
MH  - Hypersensitivity, Delayed/complications/immunology/*metabolism
MH  - Immunosorbent Techniques
MH  - Leukocytosis/etiology
MH  - Lymphokines/*metabolism
MH  - *Macrophages
MH  - Skin/*pathology
MH  - Time Factors
PMC - PMC1916088
EDAT- 1982/08/01 00:00
MHDA- 1982/08/01 00:01
PMCR- 1983/02/01
CRDT- 1982/08/01 00:00
PHST- 1982/08/01 00:00 [pubmed]
PHST- 1982/08/01 00:01 [medline]
PHST- 1982/08/01 00:00 [entrez]
PHST- 1983/02/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1982 Aug;108(2):171-83.