PMID- 6767782
OWN - NLM
STAT- MEDLINE
DCOM- 19800627
LR  - 20131121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 124
IP  - 5
DP  - 1980 May
TI  - Monohydroxyeicosatetraenoic acids (HETEs) induce degranulation of human 
      neutrophils.
PG  - 2100-4
AB  - 5-S-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE), a lipoxygenase product in 
      neurophils, and 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE), a 
      lipoxygenase product in platelets, were prepared biosynthetically and incubated 
      with purified human peripheral neutrophils. Both 5-HETE and 12-HETE at 5- to 
      10-microM concentrations induced degranulation of specific granules. Both 5-HETE 
      and 12-HETE are chemotactic for neutrophils at these concentrations. Arachidonic 
      acid, the metabolic precursor of 5-HETE and 12-HETE, did not induce 
      degranulation. The calcium ionophore A23187 induces neutrophils degranulation and 
      also activates arachidonic acid metabolism in neutrophils resulting in 5-HETE 
      production. Both ionophore-induced 5-HETE production and ionophore-induced 
      degranulation can be inhibited, with very similar inhibition curves, by 
      5,8,11,14-eicosatetraynoic acid, an inhibitor of arachidonate metabolism. We 
      propose that the mechanism for 5-HETE- and 12-HETE-induced degranulation is an 
      alteration in the fatty acid composition of membrane phospholipids.
FAU - Stenson, W F
AU  - Stenson WF
FAU - Parker, C W
AU  - Parker CW
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Arachidonic Acids)
RN  - 1191-85-1 (5,8,11,14-Eicosatetraynoic Acid)
RN  - 37H9VM9WZL (Calcimycin)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - 5,8,11,14-Eicosatetraynoic Acid/biosynthesis/pharmacology
MH  - Arachidonic Acids/metabolism/*pharmacology
MH  - Calcimycin/pharmacology
MH  - Cell Aggregation
MH  - Cell Movement
MH  - Cytoplasmic Granules/*metabolism
MH  - Humans
MH  - Muramidase/metabolism
MH  - Neutrophils/enzymology/*metabolism
MH  - Phagocytosis
EDAT- 1980/05/01 00:00
MHDA- 1980/05/01 00:01
CRDT- 1980/05/01 00:00
PHST- 1980/05/01 00:00 [pubmed]
PHST- 1980/05/01 00:01 [medline]
PHST- 1980/05/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1980 May;124(5):2100-4.