PMID- 6770034
OWN - NLM
STAT- MEDLINE
DCOM- 19800815
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 46
IP  - 2
DP  - 1980 Feb
TI  - Radioimmunoassay of measles virus nucleocapsid antigen.
PG  - 325-33
AB  - A double antibody competitive binding radioimmunoassay (RIA) was developed as a 
      tool for investigating the involvement of measles virus in persistent virus 
      infections. The assay employs 125I-labelled measles virus nucleocapsids as the 
      labelled antigen. Nucleocapsids were purified from cytoplasmic extracts of 
      virus-infected Vero cells, treated with trypsin to prevent clumping and iodinated 
      to a specific activity of about 15 microCi/microgram. Electrophoretic analysis of 
      iodinated trypsin-treated nucleocapsids revealed only labelled polypeptide with a 
      relative mol. wt. (Mr) 40 000, indicating that trypsin had cleaved the 60 000 
      mol. wt. native polypeptide to a 40 000 mol. wt. subunit. The assay could detect 
      as little as 0.1 ng of nucleocapsid protein. Either native (Mr 60 000) or cleaved 
      Mr 40 000) nucleocapsid polypeptide was detected in the assay. As much as 100 
      micrograms of protein from uninfected Vero cells did not react in the RIA. 
      Another measure of specificity was the fact that 400 ng of purified nucleocapsid 
      protein from simian virus 5, Newcastle disease virus or canine distemper virus 
      did not react in the assay. In the RIA, nucleocapsid antigen of virus isolated 
      from subacute sclerosing panencephalitis (SSPE) was indistinguishable from that 
      of Edmonston strain measles virus, indicating antigenic homology between the 40 
      000 mol. wt. nucleocapsid polypeptide of the SSPE virus and that of measles 
      virus.
FAU - Amesse, L S
AU  - Amesse LS
FAU - Payne, F E
AU  - Payne FE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antigens, Viral)
RN  - 0 (Peptides)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/*analysis
MH  - Capsid/*immunology
MH  - Cell Line
MH  - Haplorhini
MH  - Kidney
MH  - Measles virus/*immunology
MH  - Molecular Weight
MH  - Peptides/analysis
MH  - RNA, Viral/*immunology
MH  - *Radioimmunoassay
MH  - SSPE Virus/immunology
MH  - Viral Proteins/*immunology
EDAT- 1980/02/01 00:00
MHDA- 1980/02/01 00:01
CRDT- 1980/02/01 00:00
PHST- 1980/02/01 00:00 [pubmed]
PHST- 1980/02/01 00:01 [medline]
PHST- 1980/02/01 00:00 [entrez]
AID - 10.1099/0022-1317-46-2-325 [doi]
PST - ppublish
SO  - J Gen Virol. 1980 Feb;46(2):325-33. doi: 10.1099/0022-1317-46-2-325.