PMID- 6776655
OWN - NLM
STAT- MEDLINE
DCOM- 19810116
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 17
IP  - 1
DP  - 1980
TI  - Effect of carcinogenic polycyclic aromatic hydrocarbons on mouse embryonic cells 
      in culture: induction of spindle-shaped cells.
PG  - 39-49
AB  - In cultured mouse embryonic cells (MECs) treated with benzo[a]pyrene (B[a]P), 
      there appeared unusual type of fibroblasts, spindle-shaped cells (SP cells), 
      which were characterized by their narrow bipolar shape, long cellular processes 
      and optically distinct cell borders. Appearance of SP cells was massive and 
      irreversible. The amount of SP cells increased with increasing concentrations of 
      B[a]P, while early cytotoxicity did not. In various polycyclic aromatic 
      hydrocarbons (PAHs) tested, only potent carcinogens 
      (7,12-dimethylbenz[a]anthracene (DMBA), 3-methylcholanthrene (MCA), B[a]P, and 
      dibenz[a,e]pyrene (DB[a,e]P) induced SP cells. Among them, PAH having higher 
      Iball's index induced SP cells at lower concentration and at an earlier time. 
      Weak or non-carcinogenic PAHs including 3-hydroxybenzo[a]-pyrene (3-OH-B[a]P) did 
      not induce SP cells. alpha-Napthoflavon (alpha NF) suppressed the induction of SP 
      cell by carcinogenic PAH. SP cells did not appear spontaneously under various 
      abnormal culture conditions. These results indicate that carcinogenic PAHs induce 
      the appearance of a specific type of fibroblast, SP cells in MEC cultures in 
      accordance with their carcinogenicity.
FAU - Kamei, H
AU  - Kamei H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Benzoflavones)
RN  - 0 (Benzopyrenes)
RN  - 0 (Carcinogens)
RN  - 0 (Polycyclic Compounds)
RN  - 56-49-5 (Methylcholanthrene)
RN  - 57-97-6 (9,10-Dimethyl-1,2-benzanthracene)
RN  - 604-59-1 (alpha-naphthoflavone)
SB  - IM
MH  - 9,10-Dimethyl-1,2-benzanthracene/pharmacology
MH  - Animals
MH  - Benzoflavones/pharmacology
MH  - Benzopyrenes/pharmacology
MH  - Carcinogens/*pharmacology
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Embryo, Mammalian
MH  - Fibroblasts/cytology/*drug effects
MH  - Methylcholanthrene/pharmacology
MH  - Mice
MH  - Polycyclic Compounds/antagonists & inhibitors/*pharmacology
EDAT- 1980/01/01 00:00
MHDA- 1980/01/01 00:01
CRDT- 1980/01/01 00:00
PHST- 1980/01/01 00:00 [pubmed]
PHST- 1980/01/01 00:01 [medline]
PHST- 1980/01/01 00:00 [entrez]
AID - 0300-483X(80)90025-6 [pii]
AID - 10.1016/0300-483x(80)90025-6 [doi]
PST - ppublish
SO  - Toxicology. 1980;17(1):39-49. doi: 10.1016/0300-483x(80)90025-6.