PMID- 6780318
OWN - NLM
STAT- MEDLINE
DCOM- 19810424
LR  - 20181130
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 108
IP  - 1
DP  - 1981 Jan
TI  - Antiestrogens and ovine gonadotrophs: antagonism of estrogen-induced changes in 
      gonadotropin secretions.
PG  - 96-102
AB  - 17 beta-Estradiol (E2) was found to affect gonadotrophs in long term ovine 
      pituitary cell culture in several ways which were easily measured. It inhibited 
      basal FSH secretion and, concomitantly, stimulated LH secretion. High 
      physiological levels of E2 (10(-10) M; 27 pg/ml) were able to elicit about 70% of 
      the maximal responses, and the effects were developed fully between 6-24 h after 
      the addition of E2. In culture, the antiestrogens (AEs) tamoxifen, nafoxidine, 
      and CI-680 antagonized both the inhibitory and stimulatory effects of E2 (10(-10) 
      M) on gonadotropin secretion in a dose-dependent manner, and 10(-6) M AE was 
      fully effective. At concentrations of 10(-6) M or lower, the AEs showed no 
      significant intrinsic estrogenicity. At higher concentrations, the AEs 
      progressively altered gonadotropin secretion in an apparently nonspecific 
      fashion. Preincubations of cultures with AEs (3.3 X 10(-6) M) antagonized E2 
      action (10(-10) M) for approximately 18-24 h after the removal of AEs. Tamoxifen 
      appeared to be the most potent AE tested. In conclusion, long term ovine 
      pituitary cell cultures have provided a useful in vitro system for testing the 
      biological potencies of AE analogs. AEs appeared to act as pure estrogen 
      antagonists in ovine gonadotrophs.
FAU - Miller, W L
AU  - Miller WL
FAU - Huang, E S
AU  - Huang ES
LA  - eng
GR  - HD-10773/HD/NICHD NIH HHS/United States
GR  - RR-07071/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Estrogen Antagonists)
RN  - 0 (Gonadotropins)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Animals
MH  - Estradiol/pharmacology
MH  - Estrogen Antagonists/*pharmacology
MH  - Female
MH  - Follicle Stimulating Hormone/metabolism
MH  - Gonadotropin-Releasing Hormone/pharmacology
MH  - Gonadotropins/metabolism/*pharmacology
MH  - Luteinizing Hormone/metabolism
MH  - Pituitary Gland/metabolism
MH  - Sheep
MH  - Tamoxifen/pharmacology
EDAT- 1981/01/01 00:00
MHDA- 1981/01/01 00:01
CRDT- 1981/01/01 00:00
PHST- 1981/01/01 00:00 [pubmed]
PHST- 1981/01/01 00:01 [medline]
PHST- 1981/01/01 00:00 [entrez]
AID - 10.1210/endo-108-1-96 [doi]
PST - ppublish
SO  - Endocrinology. 1981 Jan;108(1):96-102. doi: 10.1210/endo-108-1-96.